Primarily supports legacy methods. BioSuite SEC Columns are available in 4.6 mm, 7.5 mm, and 7.8 mm i.d. analytical and 21.5 mm i.d. preparative and guard columns.
Chemistry |
Diol (OH) |
Separation Mode |
SEC |
Particle Substrate |
Silica |
pH Range Min |
2.5 pH |
pH Range Max |
7.5 pH |
Maximum Pressure |
1050 psi (72 bar) |
Endcapped |
Yes |
Molecular Weight Range Min |
5000 |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Parker-style |
Pore Size |
125 Å |
Format |
Column |
System |
HPLC |
USP Classification |
L59 |
Inner Diameter |
7.8 mm |
Length |
300 mm |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
BioSuite |
Product Type |
Columns |
Units per Package |
1 pk |
BioSuite Diol (OH) Column, 125Å, 5 µm, 7.8 mm X 300 mm, 5K - 150K, 1/pk
Depend on the BioSuite Diol (OH) Column to deliver maximum separation efficiency and meet the demanding challenges of protein and peptide analysis. The lab equipment uses High Resolution (HR) packing, which is a wettable silica-based material that is rigid and gives chemical stability across the 2.5 to 7.5 pH range. The BioSuite Diol (OH) Column’s length and particle size increase its separation efficiency. The column is well suited for the analysis of spherical 5 µm particles in the molecular weight range of 5,000-150,000 Daltons.
The BioSuite Diol (OH) Column has been designed to meet the challenges of protein and peptide analysis. The analytical column’s chemistry makes it the perfect lab equipment to be used for the isolation, analysis, and characterization of proteins and peptides. To ensure that it meets the demanding standards of chromatographic analysis and maximum product quality, every column goes through a rigorous, individual testing process.
You can leverage the BioSuite Diol (OH) Column in order to determine the resolution and recovery of proteins and peptides and yield excellent results. Solve your protein separation and characterization challenges with the BioSuite Diol (OH) Column to develop successful protein-based biopharmaceuticals and diagnostic reagents by adequately characterizing these complex biomolecules. Using the orthogonal separation techniques offered by the BioSuite Diol (OH) Column, you can produce the next generation drug as it helps to provide the critical characterization data and isolated material required.
Shop for lab equipment and see our latest offerings in our catalog and website. You may also want to read about the BioSuite Diol (OH) Guard Column, 5 µm, 6 mm X 40 mm, 1/pk, which has been designed by the scientists at Waters to ensure that any harmful chemical or particulate contaminants are removed from the mobile phase, and that your analytical column is protected. The Guard column is made using identical packing materials that are used in the analytical column, making it ideally suited to work in conjunction with the column and provide reliable results. By preventing any harmful particles from entering the mobile phase, the guard column also extends the lifetime of the BioSuite Diol (OH) Column.
How Is Protein Mass Calculated?
To determine the molecular weight of a protein, one can employ standard calculations by summing up the individual molecular weights of its constituent amino acids. This approach enables the accurate calculation of the protein's overall molecular weight.