Atlantis Silica HILIC Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk

Traditional reversed-phase chromatography makes it challenging to retain and separate highly basic polar analytes. Because of their hydrophilic nature and net positive charge at acidic pH, this occurs. The Atlantis Silica HILIC Column is based on HILIC, or Hydrophilic Interaction Chromatography, which is an alternate separation technique for separating water-soluble, basic, polar organic molecules. Peptides, metabolites, and actives are examples of these molecules.

The Atlantis HILIC Silica columns are used in conjunction with high organic mobile phases (>80% acetonitrile) to retain analytes that are simply too polar for reverse-phase chromatography. In comparison to traditional chromatographic reversed-phase approaches, HILIC separation mechanisms provide orthogonal analyte selectivity.

In comparison to reversed-phase HPLC columns, the Atlantis Silica HILIC Column provides better LC/ESI-MS response, direct compatibility with SPE solvents, and complementary selectivity. As metabolites are often more polar than their parent chemicals and present at substantially lower concentrations in samples, this leads to results with higher sensitivity and lower detection limits, which is extremely beneficial in drug metabolism research.

In addition to this, the Atlantis Silica HILIC Column is universally compatible with all LC detectors and offers column-to-column repeatability. Peak tailing for polar bases is common when utilizing typical chromatographic reversed-phase columns; however, the Atlantis Silica HILIC Column gives improved peak morphologies for polar bases, resulting in faster and easier technique development.

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We recommend you also look into the Atlantis Silica HILIC VanGuard Cartridge, 100Å, 5 µm, 3.9 mm X 5 mm, 3/pk; Atlantis HILIC VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 3.9 mm and 4.6 mm I.D. Atlantis HILIC analytical columns containing 5 µm sorbent particles.