Atlantis Silica HILIC Column, 100Å, 3 µm, 4.6 mm X 50 mm, 1/pk

Employ the Atlantis Silica HILIC Column in combination with high organic mobile phases (>80% acetonitrile) in order to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. The Hydrophilic Interaction Chromatography (HILIC) separation mechanism, which is the distinctive feature in the Atlantis Silica HILIC Column, provides orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches. The lab equipment enables the easy separation of water-soluble basic organic compounds, including peptides, metabolites, and actives.

Apart from offering the exceptional retention of very polar compounds, HILIC offers improved LC/ESI-MS response, as well as direct compatibility with SPE solvents. This means you get results that exhibit higher sensitivity and lower limits of detection. This is especially useful in drug metabolism studies as metabolites are often more polar than their parent compounds and are present in a much lower concentration.

When compared to reversed-phase HPLC columns, the Atlantis Silica HILIC Column also offers complimentary selectivity. Due to their hydrophilic nature combined with their net positive charge at acidic pH, the retention and separation of very basic polar analytes are extremely difficult using reversed-phase chromatography. These challenging separation problems are resolved by HILIC, making the Atlantis Silica HILIC Column the ideal lab equipment for use in metabolism, drug discovery, and combinatorial chemistries.

Benefit from the long column lifetime provided by the Atlantis Silica HILIC Column. When using the Atlantis Silica HILIC Column, you also get universal compatibility with all LC detectors. This is in addition to outstanding column-to-column reproducibility offered by the lab equipment. In addition to this, you get superior peak shapes for polar bases while leveraging faster and easier method development, in contrast to the traditional reversed-phase HPLC columns, which show a severe peak tailing for polar bases.

Learn more about HILIC chemistry through our website, which also allows you to shop for lab equipment directly from us. We recommend that you also explore and purchase the Atlantis Silica HILIC VanGuard Cartridge, 100Å, 3 µm, 3.9 mm X 5 mm, 3/pk, which is made using the identical packing materials of the Atlantis Silica HILIC Column and has been designed to enhance the column’s performance and lifetime.