Atlantis Silica HILIC Column, 100Å, 5 µm, 2.1 mm X 150 mm, 1/pk

Easily separate and retain very polar, water-soluble basic organic compounds by leveraging the power of Hydrophilic Interaction Chromatography as provided by the Atlantis Silica HILIC Column. The Waters Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. The retention and separation of very basic polar analytes is extremely difficult using reversed-phase chromatography due to their hydrophilic nature combined with their net positive charge at acidic pH. The compounds that the lab equipment enables you to process include peptides, metabolites, and actives. Other than retaining very polar compounds, HILIC offers improved LC/ESI-MS response and direct compatibility with SPE solvents. When it is compared to reversed-phase HPLC, the HILIC technology also offers orthogonal analyte selectivity.

Get results that exhibit higher sensitivity and lower limits of detection using the Atlantis Silica HILIC Column. This is especially useful in drug metabolism studies as metabolites are often more polar than their parent compounds and are present in a much lower concentration. This is why industry applications of the lab equipment include use in metabolism, drug discovery, and combinatorial chemistries, as it allows you to retain highly polar basic analytes with volatile mobile phases that are suitable for compound ionization by ESI-MS.

Depend on the long column lifetime provided by the Atlantis Silica HILIC Column. You also get universal compatibility with all LC detectors when using the Atlantis Silica HILIC Column. This is in addition to the outstanding column-to-column reproducibility offered by the lab equipment.

When using the traditional reversed-phase HPLC columns, very often, a severe peak tailing for polar bases is observed. With the Atlantis Silica HILIC Column, you get superior peak shapes for polar bases while leveraging faster and easier method development.

Waters recommends that you also opt for the Atlantis Silica HILIC VanGuard Cartridge, 100Å, 5 µm, 2.1 mm X 5 mm, 3/pk in order to protect your Atlantis Silica HILIC Column from harm and to extend its lifetime.