Separation Mode |
SEC/GPC |
Particle Substrate |
Polymer |
Molecular Weight Range Min |
100 |
Molecular Weight Range Max |
1000 |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Parker-style |
Format |
Column |
Packing Solvent |
THF |
System |
HPLC |
Technique |
GPC |
USP Classification |
L21 |
Inner Diameter |
6 mm |
Length |
150 mm |
UNSPSC |
41115709 |
Brand |
HSPgel |
Product Type |
Columns |
Units per Package |
1 pk |
HSPgel Column, 5 µm, 6 mm X 150 mm, 100 - 1K, 1/pk
The new HSPgel columns were created especially for quick GPC analysis. They provide higher sample throughput, rapid, accurate, and precise molecular weight polymer measurements, as well as significantly lower solvent use and disposal costs. Today's higher-performance solvent management techniques have made it possible to reliably and repeatedly evaluate numerous polymers using smaller columns. As a result, total analysis times are reduced to 7 minutes or less, boosting sample throughput by up to 6 times while lowering solvent consumption each run from 35 to 50 mL to 5 mL or less.
Globally, scientists utilize GPC (Gel permeation chromatography) to analyze the molecular weight distribution of polymeric substances. Typically, GPC is performed with column sets of three or four 7.8 x 300 mm columns arranged in series. For reliable and exact molecular weight measurements, the pore volume and molecular weight resolution are sufficient as a consequence. At 1.0 mL/minute eluent flow rates, the average run periods for these 3–4 column banks are 30–50 minutes. Although system band broadening and the lesser precision of earlier technologies, solvent delivery systems are minimized by the employment of these big lab equipment banks.
You can now opt for the new Waters HSPgel columns, which are specifically designed for high-speed GPC analysis. The lab equipment offers fast, accurate, and precise molecular weight polymer determinations as well as increased sample throughput and greatly reduced solvent consumption and disposal costs.
For the Waters HSPgel Column, a Certificate of Analysis and Performance Test Chromatogram are offered in the column box. We do not advise using a guard column with the lab apparatus because it can significantly increase system volume and band spreading. To prevent any particulate matter from potentially clogging the column frit, a 0.5 µm in-line filter (pre-column) can be utilized in place of the guard column.
Visit our website to shop for lab equipment and ensure your lab needs are met as needed. We also recommend considering the LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk. All Waters vials are LCMS Certified and are created using strictly regulated manufacturing and handling techniques to guarantee their inertness and freedom from pollutants and impurities. The vials undergo mass spectrometric testing, and they are delivered with a certificate of analysis that lists the manufacturing lot's reference and vial scan.
How Does One Describe HPLC?
High-performance liquid chromatography (HPLC) is an analytical technique utilized to separate, identify, and quantify components in mixtures. This method finds extensive application in various fields and stands as the most frequently employed procedure in laboratories across the globe.