Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
300 Å |
Format |
Column |
Surface Area |
110 |
System |
HPLC |
USP Classification |
L26 |
Inner Diameter |
2.1 mm |
Length |
50 mm |
Carbon Load |
2.8 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 50 mm, 1K - 30K, 1/pk
The industry standard for HPLC drug testing is still symmetry columns, offering you the assurance that your HPLC procedures will remain compliant throughout time. There's no need to sacrifice peak shape, selectivity, column-to-column and batch-to-batch consistency, or other essential performance parameters. Particle sizes, chemistries, and diameters are all available.
When compared to conventional columns of similar chemistry, the Symmetry C4 Column provides improved peak shape and peak capacity for the separation of physiologically active peptides. The Symmetry C4 Column outperforms other columns when it comes to separating hydrophilic and hydrophobic peptides, as well as neutral and highly basic peptides.
The Symmetry C4 Column will outperform other columns in high and low mass circumstances. For mass spectrometry applications, low TFA mobile phases or 0.1% formic acid are advised. Regardless of whether or where the method is used, the analytical column provides the finest answer for validation compliance.
The Symmetry C4 Column provides the sharpest peaks for biomolecules as well as increased peak capacity for complicated tryptic maps. This is made feasible by the use of ultrapure silica that is produced. The end result is a high-purity analytical column with very little silanol activity. The column is additionally packed to provide the highest efficiency and reproducibility of any product on the market, providing you with better precision for impurity and stability tests, more accurate quantitation, and adaptation to more challenging procedures.
You may be confident in the long-term compliance of your HPLC methods when using the Symmetry C4 Column. It was designed to be the gold standard for HPLC drug testing. The lab equipment allows crucial analysis to be carried out without sacrificing peak shape, selectivity, column-to-column and batch-to-batch consistency, or other important performance characteristics. Because of its sensitive, durable, and tough HPLC testing, the wide-pored, reversed-phase Symmetry C4 Column is a preferred choice in the pharmaceutical industry. Its C4 chemistry is particularly suited to large peptides and proteins.
Browse our brochure or visit our website to quickly shop for lab equipment that meets the needs of your laboratory. You may also be interested in the Symmetry C4 VanGuard Cartridge, 300Å, 5 µm, 2.1 mm X 5 mm, 1K - 150K, 3/pk; Symmetry 300 C4 VanGuard Cartridges are used to enhance analytical column lifetime and performance by eliminating particle pollution from the mobile phase stream. This cartridge is designed to protect all Symmetry 300 C4 analytical columns with 2.1 mm and 3.0 mm I.D. and 5 µm sorbent particles.
What Are The Molecular Weight Ranges That The Symmetry C4 Column Can Support?
You can use between 1000 to 30,000 molecular weight range with the Symmetry C4 Column, alongside a pH range of 2 to 8.