SCX Start Up Kit: BioResolve SCX mAb Column (2.1 mm X 100 mm) with VanGuard FIT plus mAb Standard plus pH Concentrates

Invest in the SCX Start Up Kit: BioResolve SCX mAb Column (2.1 mm X 100 mm) with VanGuard FIT plus mAb Standard plus pH Concentrates to be able to conduct analysis straight away as it offers the complete analysis package.

The monoclonal antibody (mAb) charge variations, as well as other biopharmaceutical treatments, are specially analyzed by the column to help with HPLC, UHPLC, or UPLC analysis. Hardware for the BioResolve SCX mAb column and VanGuard FIT Cartridges is made to be corrosion resistant and to reduce any negative effects that might arise from using buffers and eluents that are frequently used. To reduce diffusion-based kinetics of big macromolecules, such as mAbs, the column and VanGuard FIT Cartridge both use the same polymer-based, non-porous particles (e.g.150,000 Da). With a gradient particle structure and multi-layered surface chemistries applied to 3 m, non-porous particles, charge-based separations with improved resolution can be achieved in either LC or LC-MS applications.

A wide variety of monoclonal antibodies are frequently separated properly and consistently with charge variant quantification using strong cation-exchange analysis. However, a chromatography column that can easily tolerate chromatographic conditions is required for SCX analysis to be successful. In order to assist you in getting through those difficulties, the BioResolve Column provides the flexibility and rigidity you need. And because this lab equipment offers charge variation analysis of mAbs and other proteins, we provide LC-MS users with a platform that uses strong salt and pH gradient techniques to boost your analyses.

The non-porous polymeric particles inside the BioResolve SCX mAb Column actively strive to reduce analyte band spread to increase mAb separations, which also gives you superior component recovery, peak shape, and component resolution. The column has been set up to have a low injection-to-injection carryover rate during method transfer. Additionally, this column is LC system independent, allowing for unrestricted use on all UHPLC, HPLC, and UPLC systems. You can repeatedly obtain strong and high-resolution mAb separations in this method.

You may also be interested in purchasing the mAb Charge Variant Standard to be able to properly benchmark LC-MS system performance before performing mAb separations. When using the BioResolve SCX columns, this standard is specifically made to assist in benchmarking charge variant chromatography. It includes a special combination of whole proteins important to Biopharma's clients, including tryptophan, conalbumin, and the NIST RM 8671. (NIST mAb). Each batch of BioResolve SCX mAb materials is also quality-controlled using this standard.