XBridge Protein BEH SEC, 200 Å Column, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This PN iincludes one vial the BEH200 SEC Protein Std at no additional cost (PN 186006518).
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
6,500 psi (448 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
300 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 7.8 mm X 300 mm, 1/pk and BEH200 SEC Protein Standard Mix
The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating utilized in our popular series of HPLC-based SEC columns form the foundation of the XBridge Protein BEH SEC 200 pore size columns, which contain 2.5µm particles. This lab equipment works best when separating proteins with a size between 10,000 and 450,000 Daltons. This P/N includes one vial, the BEH200 SEC Protein Std, at no additional cost (PN 186006518). Invest in the combo kit that includes the BEH200 SEC Protein Standard Mix and XBridge Protein BEH SEC Column so you can perform analyses and make sure your system is producing the best results.
You can benefit from the easy migration approach to UltraPerformance LC technology by utilizing the XBridge Protein BEH SEC Column. Due to its unrivaled column longevity and versatility to operate over an unmatched range of mobile phase pH and temperatures, the column is a crucial piece of lab equipment. Thyroglobulin, IgG, BSA, Myoglobin, and Uracil are the five proteins that make up the BEH200 SEC Protein Standard Mix, which is part of this listing. This benchmark is meant to compare the performance of the XBridge Protein BEH SEC Column to the ACQUITY UPLC BEH200 SEC, 1.7 µm Columns. For benchmarking column and system performance, similar to the QCRM product line, frequent use of this standard is advised.
Because it was created expressly for this use, you can rely on the XBridge Protein BEH SEC Column to achieve maximum productivity. The XBridge Column is capable of handling it, whether your end goal is to produce a cutting-edge LC/MS assay or a quality control approach. The column enables longer column lives because it improves pH stability. Additionally, by enhancing particle efficiency, it provides good peak shape and peak capacity while leveraging assay robustness and improving column reliability.
You may also want to explore the XBridge Protein BEH SEC Guard Column, 200Å, 3.5 µm, 7.8 mm X 30 mm.
Across What pH Does The XBridge Protein BEH SEC Column Operate?
The XBridge Protein BEH SEC Column listed here has a minimum pH of 2.5 and a maximum of 8 pH.
What Are The Temperature Limits Of The XBridge Protein BEH SEC Column?
The XBridge Protein BEH SEC Column listed here can operate with a maximum temperature of up to 60 C.