XBridge Protein BEH SEC, 200 Å Guard Column, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This PN iincludes one vial the BEH200 SEC Protein Std at no additional cost (PN 186006518).
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
300 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Guard Column, 200Å, 2.5 µm, 7.8 mm X 30 mm, 1/pk and BEH200 SEC Protein Standard Mix
The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful range of HPLC-based SEC columns are employed in the XBridge Protein BEH SEC, 200 Guard Column, comprising 2.5um particles. They are ideally suited for separating proteins with a size range of 10,000 to 450,000 Daltons.
The XBridge Protein BEH SEC Column was designed to fulfill even the most stringent chromatographic specifications. With the XBridge Protein BEH SEC Column, you may obtain unique, mobile-phase pH stability, which helps to increase column lifetime. The XBridge Protein BEH SEC Column was created to boost lab productivity, and these multi-purpose columns can be utilized to build a quality control procedure or to establish a cutting-edge LC-MS assay.
With the XBridge Protein BEH SEC Column, you will have great column durability in your system, ensuring the robustness of assays and providing unequaled peak particle shape and capacity with exceptional efficiency. The XBridge Protein BEH SEC Column comes with a vial of the BEH200 SEC Protein Standard Mix.
Waters equipment is subjected to a variety of evaluations and individual tests in our dedicated facilities to ensure that you receive high-quality products for your laboratory and that you can trust the equipment to give precision-focused results. These facilities follow stringent industrial processes to ensure that the highest quality standards are fulfilled and equipment is built in accordance with ISO9001 and cGMP norms.
Visit our website to explore our complete line of XBridge columns and to shop for lab equipment that matches your individual needs. Please contact a member of our global team if you require assistance with any equipment or have any questions.
You might also want to look into TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; TruView LCMS Certified vials are manufactured using highly controlled manufacturing and handling techniques. Mass spectrometers are used to check for cleanliness, and ACQUITY/MS/MS is used to check for low analyte adsorption.
Can The XBridge Protein BEH SEC Column And BEH200 SEC Protein Standard Mix Be Used In What pH Range?
The XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix work well in the 2.5 - 8 pH range, at 60 C, and at a maximum pressure of 6,500 psi (448 Bar). However, we do not recommend exceeding these pH, pressure, or temperature ranges because it may have an influence on the performance and durability of your column.