BioResolve RP mAb Columns contain 2.7-μm superficially-porous-silica particles having an average pore diameter of 450Å. The particles are functionalized with a proprietary polyphenyl bonding process that was specifically designed for LC or LC-MS analyses of intact IgG monoclonal antibodies and their fragments. This P/N also includes one vial of the Intact mAb Mass Check Standard (P/N 186006552) and one vial of the mAb Subunit Standard (P/N 186008927).
Separation Mode |
Reversed Phase |
Particle Size |
2.7 µm |
Pore Size |
450 Å |
UNSPSC |
41115709 |
Brand |
BioResolve |
Product Type |
Columns |
Units per Package |
1 pk |
BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 1 mm X 100 mm, 1/pk with Intact mAb and mAb Subunit Standards
The BioResolve RP mAb Columns comprise 2.7-μm superficially porous silicon dioxide particles with an average pore diameter of 450-Å. A unique polyphenyl bonding technique that was created especially for LC or LC-MS analysis of intact IgG monoclonal antibodies and their fragments is used to functionalize the particles. Moreover, this P/N contains one vial each of the mAb Subunit Standard (P/N 186008927) and the Intact mAb Mass Check Standard (P/N 186006552).
Whole monoclonal antibodies (mAbs), mAb subunits, and antibody-drug conjugates can all be analyzed using high-quality reversed-phase chromatography (LC) or LC-MS using the BioReserve RP mAb Polyphenyl Columns (ADCs). This is made possible by using solid core particles made of silica with a well-defined 450 pore coverage and polyphenyl ligand bonding.
Each unit of the BioReserve RP mAb Polyphenyl Columns undergoes efficiency testing as well, and a Performance Test Chromatogram and Certificate of Analysis are connected to an eCord. Without an eCord reader, users of non-Waters LC systems can still access this comprehensive information. The Certificate of Analysis for each batch of packing material contains the batch number, unbonded particle analysis, bonded particle analysis, chromatographic results, and conditions.
Feel free to shop for lab equipment according to your needs on our website to find additional Waters products and collections. If you have any questions or issues, you may contact a member of our global support team on our website. They can also help you choose the appropriate products for your chromatographic and laboratory needs.
Besides the BioResolve RP mAb Polyphenyl Column, you may also be interested in Intact mAb Mass Check Standard; MS analysis of intact proteins is a potent method for assigning protein modifications and identifying proteins. An LC/MS standard used as a qualitative tool for intact mass analysis is the Intact mAb Mass Check Standard. For higher molecular weight intact mass measurements, this intact mouse protein with a known molecular weight that was purified by Protein-A is the best choice. It's given in a Waters Max Recovery Vial with the part number 18600327c. Performance testing, system setup, and routine MS tuning are all intended uses for this standard.
What Does Chromatography Achieving High Resolution Mean?
Peak width and retention time are two characteristics of the chromatographic process that are intrinsic to resolution, and as a result, resolution measurements can be utilized to optimize separations. Hence, better and more accurate results may be examined the greater the resolution attained.