Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Temperature Limits |
45 C |
Endcapped |
Yes |
Bonding Technology |
T3 |
Silanol Activity |
Medium |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
1.8 µm |
Endfitting Type |
Parker-style |
Pore Size |
100 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
230 |
System |
UHPLC, UPLC |
Particle Technology |
HSS |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
150 mm |
Carbon Load |
11 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Peptide HSS T3 C18 Column, 100Å, 1.8 µm, 2.1 mm x 150 mm, 1/pk with Cytochrome C Digestion Standard
To harness the power of UPLC technology using reverse-phase techniques, you need a UPLC column that’s ready for the difficult task. But with the ACQUITY UPLC Peptide HSS T3 C18 Column, you’ll get an UPLC column that will change the way you perform peptide and protein analysis forever. This column is uniquely suited for the separation of proteins by reverse-phase UPLC, making it a top choice for scientists working within that mode. What’s more, all ACQUITY UPLC Columns come equipped with MaxPeak High-Performance Surface technology that actively prevents unwanted analyte-surface interactions from occurring. Add the ACQUITY UPLC Peptide HSS T3 C18 Column to your lab’s toolkit and get back to the important proteomics assays that matter.
The ACQUITY UPLC Peptide HSS T3 C18 Column comes with a Cytochrome C Digestion Standard as well. This is included because all LC-MS systems should be checked for performance, especially when using UPLC equipment for peptide-related assays. Use the Cytochrome C Digestion Standard to ensure proper performance of your UPLC system before performing separations. This will help you make calibrations to your equipment before proceeding.
Another major benefit of owning the ACQUITY UPLC Peptide HSS T3 C18 Column is that it offers a wide and usable pH range to accommodate varying analytes. Plus, because UPLC systems subject columns to extreme backpressures and other trying conditions, this column has been designed to withstand and overcome those extreme conditions. You need only look at the ACQUITY UPLC Peptide HSS T3 C18 Column’s maximum pressure limit to see that this column is more than capable of taking on demanding UPLC conditions.
Protecting your UPLC column from chemical fouling should always be a top priority when you’re hard at work in the lab. To streamline the column protection process, we’re happy to provide the ACQUITY UPLC Peptide HSS T3 VanGuard Pre-column. This pre-column removes particulate contaminants from the mobile phase stream before they enter your analytical column. Thanks to this, you can enjoy longer column lifetimes.
When you shop lab equipment at Waters, you’ll see a truly massive selection of products and educational resources to help you in the lab. No matter if you need quality control reference materials, lab informatics or classic chromatography consumables, we truly have them all. Turn to us for all your lab equipment needs.
What’s the ACQUITY UPLC Peptide HSS T3 C18 Column’s Carbon Load?
The ACQUITY UPLC Peptide HSS T3 C18 Column offers an 11% carbon load.