XBridge Protein BEH SEC, 200 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This P/N also contains one vial the BEH200 SEC Protein Std (P/N 186006518) at no additional cost.
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
3200 psi (221 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
500000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
HPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
300 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 300 mm, 1/pk and BEH200 SEC Protein Standard Mix
Invest in the combination pack of the XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix so you can conduct analysis and also ensure that your system is delivering the optimum results.
The XBridge Protein BEH SEC 200 Å pore size columns, containing 3.5um particles, are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. These lab equipment are best suited to separate proteins ranging from 10,000 – 450,000 Daltons.
The BEH200 SEC Protein Standard Mix included in this listing is a 5 component protein mixture that includes Thyroglobulin, IgG, BSA, Myoglobin, and Uracil. This standard is used to benchmark ACQUITY UPLC BEH200 SEC, 1.7 µm Columns, as well as the XBridge Protein BEH SEC Column. Regular use of this standard is recommended for benchmarking column and system performance, similar to the QCRM product line.
By using the XBridge Protein BEH SEC Column, you can leverage the seamless migration method to UltraPerformance LC technology. The column is an essential piece of lab equipment as it provides unmatched column lifetime and the flexibility to work over an unparalleled range of mobile phase pH and temperatures.
Depend on the XBridge Protein BEH SEC Column to deliver maximized productivity as it is designed specifically for this purpose. Whether your end goal is to create a quality control method or to develop a cutting-edge LC/MS assay, the XBridge Column will be able to handle the requirement. The column offers improved pH stability and, therefore, provides extended column lifetimes. In addition to this, it enables you to leverage assay ruggedness and enhance column reliability, while offering excellent peak shape and peak capacity by maximizing particle efficiency.
Shop for lab equipmentt such as the combination pack listed here and more that fulfill your analytical needs. You may also want to explore the XBridge Protein BEH SEC Guard Column, 200Å, 3.5 µm, 7.8 mm X 30 mm.
What Is Protein Chromatography Used For?
Chromatography can be relied upon to separate protein in solution or denaturing conditions by using porous gels. This technique is known as size exclusion chromatography. The principle is that smaller molecules have to traverse a larger volume in a porous matrix.