XBridge Protein BEH SEC, 125 Å Column, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 1,000 – 80,000 Daltons. Note: This P/N also contains one vial the BEH125 SEC Protein Std (P/N 186006519) at no additional cost.
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
3200 psi (221 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
80000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
125 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
HPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 125Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH125 Protein Standard Mix
Use the XBridge Protein BEH SEC Column to easily design and transfer SEC methods based on laboratory apparatus. The lab equipment also allows you to create methods based on the resolution of required protein components and sample throughput requirements. The XBridge Protein BEH SEC Columns were developed specifically for size-exclusion chromatography of proteins to supplement Waters' existing line of smaller particle size, UPLC-based SEC columns.
The 125 Å XBridge Protein BEH SEC Column, containing 3.5um particles, is based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. The lab equipment is best suited to separate proteins ranging from 1,000 to 80,000 Daltons. This particular listing also contains one vial, the BEH125 SEC Protein Std (P/N 186006519), at no additional cost.
The XBridge Protein BEH SEC Column offers a longer column life than silica-based SEC columns because it has fewer unwanted protein-column interactions. The lab equipment has better throughput and delivers HPLC-based SEC resolution of proteins from 1,000 – 80,000 Daltons. The flow and pressure tolerance of these diol-bonded XBridge Protein BEH SEC Columns allows for enhanced sample throughput on HPLC lab equipment. By combining BEH base particle technology with new diol bonding techniques, they deliver column stability, performance, and lifetime.
The XBridge Protein BEH SEC Column, like all lab equipment developed and delivered by Waters, ensures consistent and reliable performance because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities. Through maintaining unrivaled column uniformity and your confidence in validation techniques, extensive testing is performed at each level, from the packing materials to post-production.
You may also want to learn about the XBridge Protein BEH SEC Guard Column, 125 Å, 3.5 µm, 7.8 mm X 30 mm, 1/pk and BEH125 Protein Standard Mi?. The kit contains a Waters guard column that makes it possible to enhance the lifetime of the analytical column by removing unwanted particles from the mobile stream. It also includes a standard mix using which you can conduct performance benchmarking.
What Is The pH Range For The XBridge Protein BEH SEC Column?
The XBridge Protein BEH SEC Column listed here has a pH range of 2.5 - 8.