ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 1 mm X 100 mm, 3/pk

Add the ACQUITY UPLC BEH Amide Column to your lab equipment collection for excellent retention of polar analytes across a wide range of polarity (including carbohydrates and sugars), structural moiety, and PKA. Because it was designed to retain polar analytes and metabolites that are too polar for reversed-phase (RP) chromatography, the column can be utilized with a wide range of mobile phase pH. (2-11). BEH technology combined with a tri-functionally linked amide phase in the column results in a column with exceptional lifetime and assay robustness.

Choose the ACQUITY UPLC BEH Amide Column to benefit from Waters' revolutionary ethylene bridged hybrid (BEH) particle technology's chemically stable, tri-functionally connected amide phase. This offers a new degree of stability and versatility to HILIC (Hydrophilic Interaction Chromatography) separations. Unbonded BEH particles used in laboratory equipment enable for orthogonal separation of polar and ionizable chemical mixtures. With a unique design that helps to overcome one of the fundamental weaknesses of HILIC stationary phases, chemical stability, the column has been designed and tested to perform efficient, reproducible separations under UPLC HILIC settings.

When compared to reversed-phase materials, the ACQUITY UPLC BEH Amide Column provides enhanced mass spectrometry response, direct compatibility with sample preparation elutes (PPT, LLE, and SPE), and orthogonal selectivity.

Use the ACQUITY UPLC BEH Amide Column for accurate carbohydrate analysis. The 1.7 µm particle size of the column enables high-speed and high-resolution analysis in complex matrices while boosting or preserving chromatographic resolution. It also improves quantitation accuracy because, unlike amine-based columns, it does not create Schiff bases. The increased pH stability of the ACQUITY UPLC BEH Amide Column enables the utilization of high pH and high temperature to collapse, decreasing sugar anomers, lowering analysis times, and boosting MS detection.

Visit our website to shop for lab equipment, and contact us if you have any queries. ACQUITY UPLC BEH Amide VanGuard Pre-column; Amide columns, such as ACQUITY BEH, retain extremely polar compounds like carbohydrates and sugars. Based on BEH technology, the ACQUITY Amide columns have a chemically stable, tri-functionally linked amide phase that is stable from pH 2 to 12, allowing them to separate polar analytes with a wide range of polarity, structural moiety, and pKa.