SKU: 176001915
ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 1 mm X 100 mm, 3/pk

ACQUITY UPLC BEH Amide Column | 176001915


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Product Description

ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa.

Specifications

  • Chemistry

    Amide

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • Format

    Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • Inner Diameter

    1 mm

  • Length

    100 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    3 pk

Product Support

Documents

Documents



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ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 1 mm X 100 mm, 3/pk

Add the ACQUITY UPLC BEH Amide Column to your lab equipment collection for excellent retention of polar analytes across a wide range of polarity (including carbohydrates and sugars), structural moiety, and PKA. Because it was designed to retain polar analytes and metabolites that are too polar for reversed-phase (RP) chromatography, the column can be utilized with a wide range of mobile phase pH. (2-11). BEH technology combined with a tri-functionally linked amide phase in the column results in a column with exceptional lifetime and assay robustness.

Choose the ACQUITY UPLC BEH Amide Column to benefit from Waters' revolutionary ethylene bridged hybrid (BEH) particle technology's chemically stable, tri-functionally connected amide phase. This offers a new degree of stability and versatility to HILIC (Hydrophilic Interaction Chromatography) separations. Unbonded BEH particles used in laboratory equipment enable for orthogonal separation of polar and ionizable chemical mixtures. With a unique design that helps to overcome one of the fundamental weaknesses of HILIC stationary phases, chemical stability, the column has been designed and tested to perform efficient, reproducible separations under UPLC HILIC settings.

When compared to reversed-phase materials, the ACQUITY UPLC BEH Amide Column provides enhanced mass spectrometry response, direct compatibility with sample preparation elutes (PPT, LLE, and SPE), and orthogonal selectivity.

Use the ACQUITY UPLC BEH Amide Column for accurate carbohydrate analysis. The 1.7 µm particle size of the column enables high-speed and high-resolution analysis in complex matrices while boosting or preserving chromatographic resolution. It also improves quantitation accuracy because, unlike amine-based columns, it does not create Schiff bases. The increased pH stability of the ACQUITY UPLC BEH Amide Column enables the utilization of high pH and high temperature to collapse, decreasing sugar anomers, lowering analysis times, and boosting MS detection.

Visit our website to shop for lab equipment, and contact us if you have any queries. ACQUITY UPLC BEH Amide VanGuard Pre-column; Amide columns, such as ACQUITY BEH, retain extremely polar compounds like carbohydrates and sugars. Based on BEH technology, the ACQUITY Amide columns have a chemically stable, tri-functionally linked amide phase that is stable from pH 2 to 12, allowing them to separate polar analytes with a wide range of polarity, structural moiety, and pKa.

What is An Amide Column?

An Amide Column is a column with an amide stationary phase and is designed to retain extremely polar analytes that are too polar to be retained by traditional reverse-phase chromatography.