ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pk
With a focus on innovative lab equipment and stationary phases for Hydrophilic Interaction Chromatography (HILIC) to overcome the challenge of retaining and separating extremely polar compounds, ACQUITY UPLC BEH Amide Columns use the novel ethylene bridged hybrid (BEH) to enable a new dimension in stability and versatility. This is done using the chemically stable, trifunctionally bonded amide phase.
ACQUITY UPLC BEH Amide Columns facilitate the ability to achieve exceptional retention of polar analytes spanning a wide range in polarity, structural moiety and, pKa through the use of a wide range of mobile phase (2-11). In addition to the enhanced retention of polar analytes and metabolites, these columns provide increased mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), and orthogonal selectivity when compared to reversed-phase materials.
The ACQUITY UPLC BEH Amide Column is an excellent tool for the analysis of carbohydrates, such as monosaccharides, disaccharides, oligosaccharides, and polysaccharides. The increased chemical stability of BEH particles enables the use of both high pH and high temperature to collapse reducing sugar anomers, shortening analysis time, and improving MS detection. The BEH particle also provides exceptional column lifetime, improving assay robustness. Lifetime can be extended further through the use of an ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk.
Unlike the amine-based columns used for carbohydrate analysis, the ACQUITY UPLC BEH Amide column is not vulnerable to Schiff-based formation, leading to improved quantitation accuracy. The 1.7 µm particle size in these columns enables high resolution, high-speed analysis of carbohydrates in complex sample matrices.
