Columns for Antibody Drug Conjugates (ADC)

SKU: 176001907
ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pk

ACQUITY UPLC BEH Amide Column | 176001907


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Product Description

ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa.

Specifications

  • Chemistry

    Amide

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • Format

    Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    3 pk

Product Support

Documents

Documents



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ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pk

Our ACQUITY UPLC BEH Amide columns have a chemically stable, trifunctionally joined amide phase based on Waters innovative ethylene bridging hybrid (BEH) particle technology. This gives HILIC (Hydrophilic Interaction Chromatography) separations a whole new level of stability and adaptability. For combinations of polar and ionizable chemicals, the utilization of unbonded BEH particles enables an orthogonal separation mechanism.

ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology, the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable across a wide pH to enable the separation of polar analytes that span a wide range of polarity, structural moiety, and pKa.

The lab equipment is developed and proven to achieve efficient, repeatable separations under UPLC HILIC conditions, overcoming a significant shortcoming of the HILIC stationary phases, chemical stability. The ACQUITY BEH packing materials were created expressly for use with the ACQUITY UPLC System and are made in a cGMP, ISO 9001 accredited facility with ultra-pure reagents. Each batch of ACQUITY BEH packing material is chromatographically tested with acidic, basic, and neutral analytes, with results held within narrow specification ranges to ensure outstanding, repeatable performance. On the eCordTM Intelligent Chip, each column is independently examined, and a Performance Chromatogram and Certificate of Batch Analysis are provided.

When compared to reversed-phase materials, the Waters ACQUITY UPLC BEH Amide lab equipment provides enhanced mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), as well as orthogonal selectivity.

We recommend that you invest in the ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk so that it helps the lab equipment continue to perform at its optimum. The pre-column is made using the exact packing materials that are present in the column, making it an ideal fit for the analytical column.

What Is The Operating pH Range Of The ACQUITY UPLC BEH Amide Column?

The ACQUITY UPLC BEH Amide Column listed here has an operating pH range of 2 pH to 11 pH.

What Is the Maximum Pressure That The ACQUITY UPLC BEH Amide Column Can Sustain?

The ACQUITY UPLC BEH Amide Column listed here can work with up to 18000 psi (1240 Bar).