Columns for Antibody Drug Conjugates (ADC)

SKU: 176000907
ACQUITY UPLC BEH Phenyl Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pk

ACQUITY UPLC BEH Phenyl Column | 130Å | 1.7 µm | 2.1 mm X 50 mm | 3/pk | 176000907


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Product Description

ACQUITY BEH Phenyl columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds. This sorbent, along with the XBridge BEH Phenyl, provide you with the most chemiscally stable phenyl sorbent on the market and provides you with ultra-low column bleed, long column lifetimes and excellent peak shape.

Specifications

  • Chemistry

    Phenyl

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    Phenyl-Hexyl

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • Format

    Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L11

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • Carbon Load

    15 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    3 pk

Product Support

Documents

Documents



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ACQUITY UPLC BEH Phenyl Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pk

Reversed-phase columns that contain phenyl-ligand are a top choice for chromatographers who need selectivities complementary to other straight-chain-alkyl stationary phases. However, these ligands traditionally have had disadvantages, including poor pH stability, reproducibility, and peak shape. The ACQUITY UPLC BEH Phenyl Column has been designed to overcome these challenges, providing complementary selectivities, excellent pH stability, and superior peak shape across compounds with one piece of lab equipment.

ACQUITY UPLC BEH Phenyl Columns use a trifunctional C6-alkyl tether between the phenyl ring and the silyl functionality. Combining this phenyl-hexyl ligand with a proprietary end-capping process allows for chromatographers to experience ultra-low column bleed, long column lifetimes, and superior peak shape. This innovative blend of ligand, end-cap, and 1.7 µm BEH particles allows for new levels of selectivity and efficiency in UPLC separations that were once more challenging.

Along with these rugged and efficient stationary phases, ACQUITY UPLC BEH Phenyl Columns have been designed to offer the longest possible lifetime in columns in UPLC conditions. The improved column hardware minimizes band broadening and ensures a connection without leaks, along with a frit design that keeps particles in the column, not the detector or the MS source. Along with the proprietary column-packing station and process, these innovative designs guarantee a stable packed column bed and long, reproducible column lifetime.

All columns are manufactured under strict processes to ensure reproducibility year over year. ACQUITY UPLC BEH Phenyl Columns are chromatographically tested in facilities compliant with industry regulation, and further testing can be performed using a Neutrals QC Reference Material from Waters.

How Should I Clean My ACQUITY UPLC BEH Phenyl Column?

Contamination of the column may be present if you notice changes in peak shape, peak splitting, shoulders on the peak, shifts in retention, changes in resolution, or increasing backpressure. If this occurs, flush your column with a neat organic solvent and take care not to precipitate buffers. If this does not solve the problem, you will need to purge the column. Purging should always be done using a routine that matches the properties of your samples and/or what you believe is contaminating the column. If performance remains poor after purging, call your local Waters office for continued support.