ProteinWorks Auto-eXpress High Digest Kit - Ambient Refill Kit
In traditionally complex LC-MS/MS protein quantification operations using the surrogate peptide technique, sample preparation is a significant source of variability. ProteinWorks eXpress Digest Kits streamline and standardize complex pre-analytic procedures for LC-MS protein quantification in whole or immunopurified plasma by streamlining and accelerating protein digestion.
The Waters® ProteinWorks Auto-eXpress Digest Kits are flexible, broadly applicable sample preparation kits with premeasured, lot-traceable chemicals created for exact, accurate, and dependable surrogate peptide-based LC-MS protein quantification. The ProteinWorks Auto-eXpress Low Digest Kit can be used to digest proteins in immuno-purified plasma or serum. The ProteinWorks Auto-eXpress High Digest, listed here, can best digest proteins in whole, non-immuno-purified plasma or serum. For the purpose of quantification, either 3-Step (without alkylation and reduction) or 5-Step (with alkylation and reduction) kits are available to accommodate a variety of proteins.
The ProteinWorks Auto-eXpress High Digest Kit - Ambient Refill Kit is utilized to directly degrade proteins in plasma or serum. This lab equipment is best used with samples that have between 1.0 mg and 5.25 mg of total protein and is suitable for sample pellet digestion following protein-level precipitation. Your lab may regularly obtain extremely repeatable, accurate, and sensitive protein measurement procedures with ProteinWorks. This adaptable and automated kit-based approach shortens the time required for method development and is simple to deploy from lab to lab and site to site.
The RapiGest denaturant vial (1x), trypsin dissolving reagent (1x), digestion inactivation reagent (1x), and premeasured, lot-traceable digestion buffer (1x) are all included in the ProteinWorks Auto-eXpress High Digest Kit - Ambient Refill Kit for high protein content samples (1.0 - 5.25 mg total protein per sample).
You may also want to explore the ProteinWorks Auto-eXpress High 3 Digest Kit. With the help of the ProteinWorks Auto-eXpress High 3 Digest Kit, complete plasma and serum samples can be quickly and easily digested for use in surrogate peptide-based LC/MS protein quantification. For the denaturation, digestion, and quenching of proteins in biological matrices, the kit provides a reliable digest technique and premeasured, lot-traceable reagents in an automation-friendly package. This lab equipment contains the High Digest Ambient Kit from ProtienWorks and High Digest Trypsin Kit from ProteinWorks and is ideal for samples with high protein content (1.0 - 5.25 mg total protein).
