IEX-MS Start Up Kit: BioResolve SCX mAb 3 µm Column (2.1 x 50 mm) plus Humanized mAb Mass Check Standard plus IonHance CX-MS pH Buffer Concentrates in MS Certified LDPE Containers

Non-porous, polymer-based particles in the BioResolve SCX mAb, 3 μm, corrosion-resistant column (2.1 x 50), are grafted with a meticulously designed, multi-component network of negatively charged sulfonic acid ligands. Using reliable salt or pH gradient approaches, this cutting-edge column technology achieves reproducible, high-resolution, charged-based separations of mAbs and other proteins in both LC and LC-MS applications. A vial of Humanized mAb Mass Check Standard (P/N 186009125), two bottles of IonHance CX-MS pH Concentrate A (pH 5.0) and B (pH 8.5), both in MS Certified LDPE Containers, are also included in this IEX-MS beginning kit.

The recommended method for characterizing biopharmaceuticals, in particular, monoclonal antibodies (mAbs), is to analyze intact proteins using LC-MS. This method allows for the quick confirmation of sequence and post-translational modifications as well as a thorough review of any product-related impurities. Waters Humanized mAb Mass Check Standard was created for this specific purpose, allowing you to compare LC-MS techniques and confirm instrument proficiency testing when performing intact protein analysis in your lab.

To increase analyte retentivity and peak form, liquid chromatography setups typically include mobile-phase additives such as strong acids or buffers. High-purity, mobile-phase additives must be added as a mandatory step for MS applications in order to minimize background contribution and guarantee data quality. Waters BioResolve SEC mAb Columns are produced in an ISO 9001- and cGMP-certified facility employing strict manufacturing procedures and ultra-pure chemicals. To ensure column-to-column uniformity, each column is then assessed for effectiveness and mAb performance using the Waters mAb Size Variant Standard.

For LC and LC-MS procedures, such as reversed-phase separations of proteins, peptides, and small compounds, ionHance modifiers are employed. To help in attaining low metal content and high-quality standards, the IonHance modifiers have been thoughtfully created. It is advised to keep the standard included with the IEX-MS Start Up Kit in its original packaging at -20 C until it has expired before reconstituting it. The standard can be stored after reconstitution for seven days at 4 to 8 C without worrying about degradation.

Please visit our website to shop for lab equipment as per your lab needs. You may also want to review the IonHance CX-MS pH Concentrate B pH 8.5 in MS Certified LDPE Container (10X Concentrate); Designed alongside the BioResolve SCX columns, the IonHance CX-MS pH 10X Concentrate B, in MS Certified LDPE Container, was carefully optimized for use in creating a gradient for LC/MS analysis of mAb charge variant separations.