ProteinWorks Auto-eXpress High 5 Digest Kit

SKU: 176004080
ProteinWorks Auto-eXpress High 5 Digest Kit

ProteinWorks Auto-eXpress High 5 Digest Kit | 176004080


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Product Description

"The ProteinWorks™ Auto-eXpress High 5 Digest Kit simplifies and accelerates the digestion of whole plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach for protein bioanalysis. The kit includes a robust digest protocol and pre-measured, lot-traceable reagents in an automation friendly format for the denaturation, reduction, alkylation, digestion and quench of proteins in biological matrices. This product is suitable for high protein content samples (1.0 - 5.25 mg total protein) and contains the following: ProtienWorks Auto-eXpress High Digest Ambient Kit (1) Digestion Buffer (1) Trypsin Dissolving Agent (1) Digestion Inactivation Agent (1) RapiGest SF Surfactant for low protein content samples ProteinWorks High Digest Trypsin Kit (1) Trypsin for low protein content samples ProteinWorks Reduction Alkylation Kit (3) Reduction Agent vials. Each vial is enough for 32 samples (3) Alkylation Agent vials. Each vial is enough for 32 samples"

Specifications

  • Number of Analysis

    96

  • Type

    Sample Preparation Workflow

  • Separation Mode

    RPLC

  • Detection Method

    MS/MS

  • System Type

    LC-MS

  • UNSPSC

    41116104

  • Application

    Peptide, Protein

  • Brand

    ProteinWorks

  • Product Type

    Application Kits

Product Support

Documents

Documents



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ProteinWorks Auto-eXpress High 5 Digest Kit

The ProteinWorks Auto-eXpress High 5 Digest Kit is a comprehensive solution designed for automated and high-throughput protein digestion in proteomic research workflows. The lab equipment offers convenience, efficiency, and reliability in sample preparation for protein identification and characterization.

Opt for the use of the ProteinWorks Auto-eXpress High 5 Digest Kit in your lab, as it provides all the necessary reagents and consumables for efficient protein digestion. The lab equipment includes optimized protocols and procedures to ensure consistent and reproducible results. The kit is specifically designed for automated liquid handling systems, allowing for high-throughput processing of protein samples. Automating the digestion process minimizes hands-on time, reduces variability, and improves workflow efficiency.

The ProteinWorks Auto-eXpress High 5 Digest Kit utilizes a proprietary combination of enzymes and buffers to achieve highly efficient protein digestion. It enables rapid and complete digestion of proteins, resulting in peptide mixtures that are suitable for subsequent mass spectrometry analysis.

This lab equipment is compatible with a wide range of sample types, including purified proteins, cell lysates, and tissue extracts. It offers flexibility in sample input amounts and is suitable for small to large-scale protein digestion experiments.

The ProteinWorks Auto-eXpress High 5 Digest Kit is optimized for compatibility with Waters' liquid chromatography and mass spectrometry systems, ensuring seamless integration into proteomic workflows.

By providing reliable and reproducible protein digestion results, this kit enables researchers to obtain high-quality data for protein identification, quantification, and post-translational modification analysis.

You may also want to learn more about the ProteinWorks µElution SPE Clean-up Kit, which has been specifically developed to facilitate the clean-up of peptides after digestion in LC-MS protein quantification using the surrogate peptide approach. This kit incorporates a meticulously optimized solid-phase extraction (SPE) protocol and utilizes Oasis uElution technology, which ensures the highest possible recovery of peptides while effectively eliminating digestion reagents and matrix interferences.

Can The Protein Works Auto-Express High 5 Digest Kit Be Used For Different Types Of Samples?

Yes, the ProteinWorks Auto-eXpress High 5 Digest Kit is designed to be compatible with a variety of sample types, including purified proteins, cell lysates, and tissue extracts.

What Is The Recommended Sample Input For The ProteinWorks Auto-Express High 5 Digest Kit?

The recommended sample input for the ProteinWorks Auto-eXpress High 5 Digest Kit can vary depending on the specific application and sample complexity. However, the kit provides flexibility in sample input amounts, accommodating a wide range of concentrations.