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Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive.
This basic kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, and the GlycoWorks Clean-up Module (excludes PN 186007988).
It contains materials to process 24 samples in a 3x8 format.
The kit contains:
GlycoWorks Deglycosylation Module:
(1) 0.035 mL/vial Rapid PNGaseF Enzyme
(1) 0.25 mL/vial Glycoworks Rapid Buffer
(1) 3 vials of 3 mg/vial RapiGest SF Surfactant
(1) Intact mAb Mass Check Standard *Shipped in a separate box
GlycoWorks RapiFluor-MS Labeling Module:
(3) 9 mg/vial RapiFluor-MS Reagent
(3) 1 ml/vial Reagent Solvent (Anhydrous DMF)
GlycoWorks RFMS Clean-up Module:
(4) 0.5 mL/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)
(4) 3 mL/vial Sample Diluent (DMF/ACN Mixture)
(1) GlycoWorks HILIC µElution Plate
Specifications
Number of Analysis
24
Type
Sample Preparation Workflow
Packages per kit
4
Separation Mode
HILIC
Detection Method
HRMS, MS, MS/MS, UV
Storage Condition
Refer to the storage recommendations of each sub-package
System Type
LC-MS
Shelf Life
Refer to the storage recommendations of each sub-package
UNSPSC
41116104
Application
Glycan
Brand
GlycoWorks
Product Type
Application Kits
Units per Package
1 pk
Cold ShippingThis part will be shipped cold and shipping and handling fees will be applied to your cart.
Invest in the Glycoworks RapiFluor-MS N-Glycan Basic Kit-24 sample so you can conduct N-glycans analysis rapidly. The compact yet effective labeling module includes all the crucial reagents required to analyze released N-glycans in a faster, more streamlined, and highly sensitive way. The Glycoworks RapiFluor-MS N-Glycan Basic Kit-24 sample essentially works by reducing complicated, time-consuming sample preparation. It delivers the desired analysis with just 3 easy steps in as little as 30 minutes.
Use the Glycoworks RapiFluor-MS N-Glycan Basic Kit-24 sample to leverage outstanding benefits for your analysis. It enables you to improve product quality understanding with in-depth characterization of previously unidentified peaks with fluorescence quantification obtained with equivalent mass spectral response. In addition to this, using the Glycoworks RapiFluor-MS N-Glycan Basic Kit-24 sample will speed up the decision-making while making it easier to identify low abundance N-linked glycans.
The kit listed here includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, and the GlycoWorks Clean-up Module (excludes PN 186007988). It contains materials to process 24 samples in a 3x8 format. The kit contains: GlycoWorks Deglycosylation Module: (1) 0.035 mL/vial Rapid PNGaseF Enzyme (1) 0.25 mL/vial Glycoworks Rapid Buffer (1) 3 vials of 3 mg/vial RapiGest SF Surfactant (1) Intact mAb Mass Check Standard *Shipped in a separate box GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent (3) 1 ml/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module: (4) 0.5 mL/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7) (4) 3 mL/vial Sample Diluent (DMF/ACN Mixture) (1) GlycoWorks HILIC µElution Plate.
The Glycoworks RapiFluor-MS N-Glycan Basic Kit-24 sample is intended to be used as a part of the GlycoWorks RapiFluor - MS N-Glycan Kit. It has unique chemical attributes that offer increased fluorescence quantification and supreme mass spectral response, meaning that you need to use only this one label as it provides valuable information from characterization to routine monitoring and that Glycan analysis is much easier and more sensitive. Shop for lab equipment and see the complete range of GlycoWorks Kits available with us. You may also want to explore the RapiFluor-MS Glycan Performance Test Standard.
How Are Glycans Isolated?
Traditionally, glycans are isolated from proteins or peptides using a combination of hydrophobic and hydrophilic columns. While proteins and peptides remain on hydrophobic absorbent, the glycans, salts, and other hydrophilic reagents are collected as flowthrough.
