Intact mAb Mass Check Standard

Intact mAb Mass Check Standard

Fully characterized monoclonal antibody standard

Fully characterized monoclonal antibody standard

Analyzing intact proteins by mass spectrometry (MS) is a powerful approach for protein identification, mAb characterization, and assignment of protein modifications.

The Intact mAb Mass Check Standard is a LC-MS reference standard used as a qualitative tool for intact mass analysis. With this standard, you can more easily and efficiently achieve your routine MS optimization, system setup and performance testing requirements – ensuring that the MS instrument is tuned and calibrated.

Waters Intact mAb Mass Check Standard is ideal for higher molecular weight intact mass measurements. Intact mAb Mass Check Standard

Specifications

Overview

  • Intact mouse IgG1 protein purified by Protein-A with a known molecular weight
  • Used as the unlabeled, control/reference standard for the GlycoWorks RapiFluor-MS N-Glycan sample preparation workflow as it has 4 major glycosylated peptides
  • Provided in a Waters TruView Vial (ideal for peptides) as 1 mg of lyophilized solid (lyophilization grade sucrose is used for stabilization)

Recommended Use: For higher molecular weight intact mass measurements.


Features Header

Features Header


Protein Information

Intact Protein Formula C6472 H9940 N1698 O2008 S52

Average MW = 145,329.7

  • 17 Disulfide Bonds 
  • N-terminus of each heavy chain has a fixed Pyroglutamic acid from Q
  • One N-linked glycosylation on each heavy chain. Total Number of Glycoforms: 4 (G0, G0F, G1F, G2F)
  • Disclaimer: I/L are exchangeable

Sequence: Heavy chain information

*QVQLKESGPG LVAPSQSLSI TCTVSGFSLL GYGVNWVRQP PGQGLEWLGM IWGDGSTDYN SALKSRITIS KDNSKSQVFL KMNSLQTDDT AKYYCTRAPY GKQYFAYWGQ GTLVTVSAAK TTPPSVYPLA PGSAAQTDSM VTLGCLVKGY FPEPVTVTWN SGSLSSGVHT FPAVLQSDLY TLSSSVTVPS STWPSETVTC NVAHPASSTK VDKKIVPRDC GCKPCICTVP EVSSVFIFPP KPKDVLTITL TPKVTCVVVD ISKDDPEVQF SWFVDDVEVH TAHTQPREEQ FNSTFRSVSE LPIMHQDWLN GKEFKCRVNS AAFPAPIEKT ISKTKGRPKA PQVYTIPPPK EQMAKDKVSL TCMITDFFPE DITVEWQWNG QPAENYKNTQ PIMDTDGSYF VYSKLNVQKS NWEAGNTFTC SVLHEGLHNH HTEKSLSHSP G

* pyroglutamic acid from Q


Sequence: Light chain information

DVLMTQTPLS  LPVSLGDQAS  ISCRSSQYIV  HSNGNTYLEW
YLQKPGQSPK  LLIYKVSNRF    SGVPDRFSGS GSGTDFTLKI
SRVEAEDLGV  YYCFQGSHVP LTFGAGTKLE  IKRADAAPTV
SIFPPSSEQL   TSGGASVVCF LNNFYPKDIN VKWKIDGSER
QNGVLNSWTD  QDSKDSTYSM SSTLTLTKDE
YERHNSYTCE ATHKTSTSPI   VKSFNRNEC



Resources

Documents

Documents


Support

Support


Related

Streamline analytical challenges associated with mAb characterizations and associated biotherapeutic drug separations with Waters BioResolve Columns and consumables.

Leave no analyte behind with Waters TruView LCMS certified glass low adsorption autosampler vials - certified/tested for analyte recovery on a UPLC-MS/MS.

Reduce complicated, time-consuming sample preparation with GlycoWorks RapiFluor-MS N-Glycan Kit without compromising sensitivity.

Offers and Discounts

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