DisQuE, QuEChERS Sample Preparation Products
QuEChERS fast and easy sample preparation for residual analysis
Rapidly and easily screening large numbers of food and agricultural samples is vital for your organization. Waters QuEChERS kits for dispersive solid-phase extraction (d-SPE) analysis make sample prep quick and easy for your multi-residue food and agricultural analysis.
Waters DisQuE d-SPE products include conveniently packaged centrifuge tubes and foil pouches containing pre-weighed extraction buffer salts for use with the Association of Official Analytical Chemists (AOAC) or European Committee for Standardization (CEN) QuEChERS sample preparation methods. The extracts can be cleaned up with a flexible selection of dispersive SPE (d-SPE) targeting specific sample matrix.
- Ready-to-use pre-weighed, ultrapure extraction salts in both centrifuge tubes and foil pouches
- Buffer salts ideal for both AOAC (2007.01) and European (CEN 15662) QuEChERS sample preparation methods
- Easy-open foil pouches allow extraction salts to be quickly transferred into tubes without spills
- Time- and cost-saving sample preparation for multi-residue analysis
- Effective, reliable and high-quality product in a convenient format
- Efficient and matrix-specific clean-up tubes to accommodate a wide variety of commodities
- Improved consistency in extraction products, lab efficiency, workflow and quality
Recommended Use: For multi-residue analysis in food and agricultural products.
Buffer salts for the use of both AOAC (2007.01) and European CEN (EN 15662) QuEChERS sample preparation methods
AOAC method buffer salts contain 1.5 g sodium acetate and 6 g magnesium sulfate
CEN method buffer salts contain 1 g trisodium citrate dihydrate, 0.5 g disodium hydrogencitrate sesquihydrate, 1 g sodium chloride, and 4 g magnesium sulfite
Pre-weighed salts are packaged in both 50-mL centrifuge tubes and foil pouches
For method-specific extraction.
A blend of SPE sorbents packaged in centrifuge tubes
2 mL and 15 mL tubes for different sample volume needs
Each combination of sorbents with optimized weight is used targeting specific agriculture samples and commodities
Each sorbent is effective in retaining/removing specific sample matrix
C18 removes very non-polar interferences, such as fats/oils
PSA removes polar interferences, including sugars and organic acids
Graphitized carbon black (GCB) effectively remove pigments such as chlorophyll and carotenoids
For sample-matrix specific clean-up.
Complete kit solutions for use of both official methods: AOAC (2007.01) and CEN (EN 15662)
Conveniently packages both extraction and d-SPE clean-up products together
For added lab value addressing the need for simple protocols that require little training to implement.
The science behind DisQuE
The DisQuE procedure, following the QuEChERS protocol, is based on an initial single-phase extraction using up to 10 g or 15 g sample with acetonitrile at 1 mL acetonitrile per 1 g of sample. A liquid-liquid partition is created by adding excess salts and buffers to the extract. After centrifugation, the acetonitrile layer containing the analytes of interest is collected. A single d-SPE step can further reduce the sample matrix and remove excess water by mixing acetonitrile extract with anhydrous MgSO4 and primary secondary amine (PSA) sorbents. Additional sorbents like C18 or graphitized carbon black (GCB) can be added as needed for specific matrix clean-up. Alternately, the acetonitrile extract could be cleaned up using specific SPE in typical formats such as cartridges and plates. The final extract can be analyzed directly by either GC-MS or LC-MS/MS with a simple dilution.
Designed to improve pesticide analysis
QuEChERS sample preparation methods were originally developed to extract pesticides from fruits and vegetables. Combining an initial salting out liquid-liquid extraction with d-SPE clean-up, these methods have been shown to be very effective for multiresidue analysis of pesticides, veterinary drugs, and mycotoxins in a wide variety of food and agricultural products.
Alternative clean-up using Oasis PRiME HLB
Typical QuEChERS d-SPE clean-up is not suitable for acidic compounds due to the presence of strong base PSA. In order to use same single analytical extraction and clean-up method to screen for acidic, neutral and basic pesticides, Oasis PRiME HLB is used following the QuEChERS extraction step.