Nano and MicroFlow LC-MS Columns
Generate high-quality peptide and protein separations
The ability to reproducibly generate high-quality peptide and protein separations is critically important in proteomics research and biopharmaceutical characterization. Waters Ethylene-Bridged Hybrid (BEH), Charged Surface Hybrid (CSH), and High Strength Silica (HSS) peptide and protein nano columns help address these demanding needs. Combining columns with low retentive trap columns results in highly efficient separations with an increased number of peak identifications.
Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems. The selected stationary phases of these analytical columns fully leverage the separation power of sub-2–µm particle technology at flow rates between 100 nL/min and 50 µL/min, withstanding 15,000 psi, and have an inner diameter between 75 µm – 300 µm. This delivers the efficiency and selectivity required for complex separations like the discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery.
Nano and MicroFlow LC-MS Columns
Specifications
Overview
Leverage the efficiency of sub-2–μm particle technology at high flow rates
Stationary phases with a variety of characteristics suitable for low abundance species, peptide mapping, protein identification, and biomarker discovery
Easy interface with LC-MS systems
Available in BEH, CSH, and HSS particle technologies
Recommended Use: For reliable nano, capillary, and microscale UPLC separations of complex peptides and proteins.
Nano and MicroFlow LC-MS Column Featured Technologies
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Easy-to-use, re-usable, fingertight fittings for nano- and microflow LC-MS -
15,000 psi (1034 bar) pressure tolerance at 90 °C
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Compatible with ACQUITY UPLC M-Class, and V-Detail Ports (10–32 threaded ports for 1/16” O.D.)
Nano and MicroFlow LC-MS Column Characteristics
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Greatly reduce the flow-path induced variability in chromatographic results
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Deliver the efficiency and selectivity required for complex separations such as discovery of low abundance species, peptide mapping, protein identification, biomarker discovery, and biopharmaceutical characterization
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Preassembled with ZenFit Connectors
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Suitable for use with the classic nanoACQUITY UPLC and ACQUITY UPLC M-Class Systems
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Designed to provide high efficiency sized-based separations for water soluble polymer characterization
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Uses BEH particle technology
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Robust separations up to 45 ̊C
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Suitable for use with the classic nanoACQUITY UPLC and ACQUITY UPLC M-Class Systems
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Nano and microflow LC-MS columns are specialized types of chromatography columns used in liquid chromatography-mass spectrometry (LC-MS) for analyzing peptides and proteins. Here's more about these columns:
Column Size: Nano columns are very narrow, typically with diameters ranging from 75 to 300 micrometers, while microflow columns have slightly larger diameters, usually between 300 and 1000 micrometers.
Flow Rates: These columns use lower flow rates compared to standard LC columns. Nano columns typically run at 200 to 1000 nanoliters per minute (nL/min), and microflow columns operate at 1 to 10 microliters per minute (μL/min).
Sample Requirements: Due to their small size and low flow rates, nano and microflow LC-MS columns need only tiny sample volumes, ranging from nanoliters to microliters, making them ideal for analyzing limited or precious samples.
Performance: These columns are designed to efficiently separate peptides and proteins, achieving high resolution and selectivity. Their small diameter and controlled flow rates help in better separation of complex mixtures.
Construction: Nano and microflow LC-MS columns are filled with specialized stationary phases like reversed-phase C18, which interact with peptides and proteins based on their hydrophobicity. They are built to withstand high pressures for optimal performance.
Applications: These columns are extensively used in proteomics and peptide analysis for tasks like biomarker discovery, protein identification, and studying post-translational modifications. They are also crucial in pharmaceutical and clinical research requiring high sensitivity and resolution.
Compatibility: Nano and microflow LC-MS columns work seamlessly with advanced mass spectrometry systems, such as Nano LC-MS and Micro LC-MS setups. They are often used together with electrospray ionization (ESI) or Nanospray ionization sources to combine liquid chromatography with mass spectrometry.
In essence, Nano and microflow LC-MS columns are specialized tools tailored for achieving precise peptide and protein separations in LC-MS analyses. Researchers in proteomics, pharmaceuticals, and biomedical sciences rely on these columns for their unique capabilities and performance in complex sample analyses.
