AccQ•Tag Columns for Amino Acid Analysis

AccQ•Tag Columns for Amino Acid Analysis

Accurate amino acid composition analysis using Waters column solutions starts here

Accurate amino acid composition analysis using Waters column solutions starts here

Obtaining accurate amino acid analysis (AAA) composition from cell culture media, protein/peptide hydrolysates, dairy and adult nutritional products, feeds and foods, and many other samples require a rigorous and specific approach. For these challenging workflows, Waters offers a comprehensive pre-column derivatization AAA solution, including columns, mobile-phase eluents, sample preparation kits, and amino acid application standards for use with UPLC, UHPLC, and HPLC instrumentation. Using Waters UPLC AAA solutions provides 2x better resolution in ⅓ of the time compared to traditional high-speed post-column derivatization methods for amino acid analysis, allowing you to achieve better results in less time.

The AccQ•Tag Ultra Columns are designed for reversed-phase separation of amino acids derivatized with AccQ•Tag Ultra reagent. Each column is QC tested by UV detection using Waters Amino Acid standard to ensure repeatable performance for your most demanding amino acids. The 1.7 µm, AccQ•Tag Ultra columns are designed for superior speed and resolution on UPLC systems. Waters provides other AccQ•Tag column solutions for both UHPLC and HPLC platforms to ensure broad compatibility.

The AccQ•Tag Ultra Column is a critical component of the UPLC Amino Acid Analysis Solution, which is an integrated and synergistic combination of instrumentation, derivatization chemistry, separation column, eluents, methods, and software.  AccQ•Tag Columns for Amino Acid Analysis
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Specifications

Overview

  • AccQ•Tag Ultra Columns provide best-in-class column efficiency for superior separation speed, sensitivity, and resolution for amino acid analysis
  • These columns are designed for reversed-phase separation of amino acid derivatives produced by the AccQ•Tag Ultra Chemistry Derivatization Kit (Learn more)
  • Each AccQ•Tag column is quality control (QC) tested using Waters amino acid standard to ensure repeatable performance for your most demanding amino acids
  • View useful workflow guidelines for the hydrolysis and analysis of amino acids from purified peptides/proteins, foods, and feeds in the Comprehensive Guide to Hydrolysis and Analysis of Amino Acids
  • Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using ACQUITY stationary phases

Recommended Use: For accurate amino acid composition analysis.

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Features Header

AccQ•Tag Ultra Column (1.7 µm) for Waters UPLC Instruments

The AccQ•Tag Ultra Column provides the fastest runs and best resolution for Amino Acid Analysis (AAA). Optimized for Waters H-Class UPLC systems, this solution integrates instrumentation, derivatization chemistry, reversed-phase columns, separation column, eluents, and methods into a seamless, synergistic workflow.

AccQ•Tag Ultra C18 Column (2.5 µm) for UHPLC*

Achieve optimal resolution and shorter run times with AccQ•Tag Ultra 2.5 µm Columns. These columns are certified for use with the AccQ•Tag Ultra Chemistry Kit and AccQ•Tag Ultra Eluents to ensure reliable amino acid analysis.

*Recommended for UHPLC systems running 9,000 psi to 12,000 psi.

AccQ•Tag Column (4 µm) for HPLC

The AccQ•Tag Amino Acid Column is designed and certified for use with the AccQ•Tag method. These reversed-phase HPLC columns separate amino acid derivatives produced by the AccQ•Fluor Reagent Kit with proven performance and consistency.

Legacy Pico•Tag Column (4 µm) for HPLC

The Pico•Tag Column for amino acid analysis is certified for use with the Pico•Tag method and reagent kit. This HPLC column provides trusted separations of amino acid derivatives produced by phenylisothiocyanate (PITC) derivatization.

AccQ•Tag Ultra and AccQ•Fluor Derivatization

The AccQ•Tag Ultra and AccQ•Fluor derivatization methods use pre-column reagents (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) to label amino acids in a simple, single-step reaction, producing highly stable, detectable adducts.

  • The AccQ•Tag Ultra Derivatization Kit is optimized for UPLC and UHPLC systems and used with 1.7 µm and 2.5 µm AccQ•Tag Ultra Columns. This kit is QC tested with UV detection.
  • The AccQ•Fluor Reagent Kit is designed for HPLC systems and used with the 4 µm AccQ•Tag Amino Acid Column. This kit is QC tested with fluorescence detection.

These kits ensure consistent labeling, streamlined workflows, and reliable results.

Let Andrew+ pipette for you

Improve accuracy, boost reproducibility, minimize errors, and reduce sample prep time with the Andrew+ Pipetting Robot. Save samples and reagents with Andrew+ through flexible sample runs using AccQ•Tag Ultra Automation Sample Preparation Kit (32, 64, 96 sample sets). Benefit from verified OneLab protocols, compatibility with additional connected devices, self-guided installation, and easy access to online troubleshooting and support.

AccQ•Tag™ Amino Acid Sample Prep - Protocol - OneLab

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Related

Amino Acid Analysis Solutions
Waters offers three distinctively reliable methods for accurate amino acid analyses. All three utilize pre-column derivatization methods followed by reversed-phase chromatography with on-line detection of well resolved peaks using either UV absorbance or fluorescence detection.

Amino Acid Analysis Standards & Kits
Accurately separate, identify, & quantify amino acids in cell culture, protein hydrolysates, foods, and feeds with Waters AccQ•Tag and AccQ•Tag Ultra Amino Acid Analysis standards and kits for HPLC, UHPLC, and UPLC.

Automation Workflow Kits and Scripts
Realize the full potential of your automation workstations with Waters sample preparation kits, and seamlessly automate your Waters workflow to gain laboratory efficiency.

BioAdvisor
Simplify the process of choosing the best chemistry solutions for your application with the Waters BioAdvisor, which enables you to select an appropriate UPLC/UHPLC or HPLC column and/or chemistry consumable for a desired application, all organized by molecule type.

Offers and Discounts

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AccQ•Tag Columns for Amino Acid Analysis

Waters' AccQ•Tag Columns are the epitome of precision when it comes to amino acid analysis, offering a comprehensive solution for the most demanding workflows. Whether you're analyzing cell culture media, protein and peptide hydrolysates, or nutritional products, these columns are designed to deliver accurate and reliable results every time.

Accurate amino acid composition analysis is crucial in many labs across fields, including biochemistry, proteomics, food science, and clinical research. The AccQ•Tag Columns are specifically engineered for reversed-phase separation of amino acids that have been derivatized with the AccQ•Tag Ultra reagent. This derivatization process enhances the chromatographic properties of amino acids, allowing for superior separation, sensitivity, and resolution.

The AccQ•Tag Ultra Columns are available in two particle sizes: 1.7 µm for use on Waters UPLC platforms and 2.5 µm for use on Waters HPLC and UHPLC platforms. Both sizes are designed to meet the rigorous demands of modern laboratories, providing rapid and reliable separation of amino acids.

One of the standout features of the AccQ•Tag Columns is their exceptional sensitivity. These columns can detect and quantify amino acids at very low concentrations, making them invaluable for researchers who require precise and accurate data. This high sensitivity is complemented by the columns' excellent selectivity, which allows for the separation of a wide range of amino acids, including both proteinogenic and non-proteinogenic amino acids. This versatility makes the AccQ•Tag Columns suitable for a wide range of applications, from determining amino acid composition in proteins and peptides to assessing the nutritional content and quality of food and beverage products.

Reproducibility is another key advantage of the AccQ•Tag Columns. Each column undergoes rigorous quality control (QC) testing using Waters Amino Acid standard, ensuring consistent performance and reliable results with every use. This level of reproducibility is essential for researchers who need to trust that their results will be consistent, no matter how many analyses they perform.

Durability is also a hallmark of the AccQ•Tag Columns. These columns are built to withstand high pressures and harsh solvents, making them suitable for even the most demanding applications. To further extend the life of your column, optional VanGuard Cartridges are available, which are specifically designed using ACQUITY stationary phases to provide prolonged column lifetimes.

The AccQ•Tag Columns are not just about performance—they are also designed with ease of use in mind. They are compatible with various derivatization methods, simplifying the sample preparation process and allowing for seamless integration into your existing workflow. Whether you're working in protein and peptide analysis, food and beverage testing, clinical research, or environmental monitoring, AccQ•Tag Columns provide the performance and reliability you need to achieve your analytical goals.

Waters' AccQ•Tag Columns are the epitome of precision when it comes to amino acid analysis, offering a comprehensive solution for the most demanding workflows. Whether you're analyzing cell culture media, protein and peptide hydrolysates, or nutritional products, these columns are designed to deliver accurate and reliable results every time.

Accurate amino acid composition analysis is crucial in many labs across fields, including biochemistry, proteomics, food science, and clinical research. The AccQ•Tag Columns are specifically engineered for reversed-phase separation of amino acids that have been derivatized with the AccQ•Tag Ultra reagent. This derivatization process enhances the chromatographic properties of amino acids, allowing for superior separation, sensitivity, and resolution. The AccQ•Tag Ultra Columns are available in two particle sizes: 1.7 µm for use on Waters UPLC platforms and 2.5 µm for use on Waters HPLC and UHPLC platforms. Both sizes are designed to meet the rigorous demands of modern laboratories, providing rapid and reliable separation of amino acids.

One of the standout features of the AccQ•Tag Columns is their exceptional sensitivity. These columns can detect and quantify amino acids at very low concentrations, making them invaluable for researchers who require precise and accurate data. This high sensitivity is complemented by the columns' excellent selectivity, which allows for the separation of a wide range of amino acids, including both proteinogenic and non-proteinogenic amino acids. This versatility makes the AccQ•Tag Columns suitable for a wide range of applications, from determining amino acid composition in proteins and peptides to assessing the nutritional content and quality of food and beverage products.

Reproducibility is another key advantage of the AccQ•Tag Columns. Each column undergoes rigorous quality control (QC) testing using Waters' Amino Acid standard, ensuring consistent performance and reliable results with every use. This level of reproducibility is essential for researchers who need to trust that their results will be consistent, no matter how many analyses they perform.

Durability is also a hallmark of the AccQ•Tag Columns. These columns are built to withstand high pressures and harsh solvents, making them suitable for even the most demanding applications. To further extend the life of your column, optional VanGuard Cartridges are available, which are specifically designed using ACQUITY stationary phases to provide prolonged column lifetimes.

The AccQ•Tag Columns are not just about performance—they are also designed with ease of use in mind. They are compatible with various derivatization methods, simplifying the sample preparation process and allowing for seamless integration into your existing workflow. Whether you're working in protein and peptide analysis, food and beverage testing, clinical research, or environmental monitoring, AccQ•Tag Columns provide the performance and reliability you need to achieve your analytical goals.

FAQs About Waters AccQ-Tag Ultra Column

1. What is the AccQ-Tag Ultra Column used for?
The AccQ-Tag Ultra Column is used for amino acid analysis in various samples, including biological fluids, food, and beverages.

2. What is the composition of the AccQ-Tag Ultra Column?
The AccQ-Tag Ultra Column is composed of a high-purity silica gel with a unique bonding chemistry that provides high resolution and sensitivity for amino acid analysis.

3. What is the recommended flow rate for the AccQ-Tag Ultra Column?
The recommended flow rate for the AccQ-Tag Ultra Column is 0.5 mL/min.

4. What is the column temperature range for the AccQ-Tag Ultra Column?
The column temperature range for the AccQ-Tag Ultra Column is 20-60°C.

5. What is the shelf life of the AccQ-Tag Ultra Column?
Answer: The shelf life of the AccQ-Tag Ultra Column is 2 years from the date of manufacture when stored at room temperature.