ACQUITY Arc Multi-Dimensional Liquid Chromatography System
Multi-dimensional technology for HPLC separations
Waters ACQUITY Arc Multi-Dimensional Liquid Chromatography (MDLC) System enables scientists to achieve significant increase in sensitivity and selectivity, eliminate unwanted interferences, and characterize the most complex samples.
With the system, your lab can perform separations that are normally incompatible with a mass spectrometer by adding a second reversed-phase separation to your experiments. The system is comprised of two (2) Quaternary Solvent Managers (QSM), a Sample Manager with Flow-Through Needle (SM-FTN), and a Column Manager. Additional hardware includes, idiverter valves that are used when choosing the 30 cm Column Heater (CH) product.
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ACQUITY Arc Multi-Dimensional Liquid Chromatography System
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Specifications
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Total system bandspread, 4s |
≤30 μL (default configuration) |
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Dwell volume (total system) |
Path 1≤1450 μL Path 2≤1150 μL |
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Gradient delay volume |
Path 1≤1050 μL Path 2≤750 μL |
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Integrated leak management |
Leak sensors, as standard, and safe leak handling |
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Quantum synchronization |
Injection synchronization between pump and injector enhances retention time reproducibility |
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Operating flow rate range |
0.001 to 5.000 mL/min, in 0.001 mL increments |
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Maximum operating range |
9500 psi up to 5.000 mL/min |
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pH range |
1 to 12.5 |
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Unattended operation |
Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software |
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Cycle time |
≤30 seconds inject-to-inject |
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Solvent capacity |
Blend up to four solvents in any combination (standard); total capacity of nine solvents with integrated solvent select valve (optional) |
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Number of fluidic paths |
Three (Path 1, Path 2, and waste), with Arc Multi-flow path technology (standard) |
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Dwell volume selection |
Automated with Arc Multi-flow path technology |
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Solvent conditioning |
Integrated vacuum degassing, four chambers |
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Gradient formation |
Low-pressure mixing, quaternary gradient |
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Gradient profiles |
11 gradient curves [including linear, step (2), concave (4), and convex (4)] |
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Check valves |
Passive check valves |
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Flow accuracy |
+/- 1.0% at 0.5, 3.0, and 5.0 mL/min |
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Flow precision |
≤0.075% RSD or +/-0.020 min SD, whichever is greater, based on six replicates [60:40 water:methanol pre-mixed; 1.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C18 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm] |
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Composition ripple |
≤0.5 mAU [mobile phase containing 0.1% TFA in water/acetonitrile; 1.5 mL/min; CORTECS C18 2.7 μm, 4.6 x 50 mm; 35 °C; UV @214 nm] |
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Composition accuracy |
+/- 0.5% absolute (full scale) from 5 to 95%; 0.5 to 5.0 mL/min [methanol; methanol with 5.0 mg/mL caffeine step gradient; UV @273 nm] |
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Composition precision |
+/- 0.15% RSD or 0.04 min SD, whichever is greater based on six replicate injections [60:40 water:methanol via Auto•Blend Technology; 0.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C18 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm] |
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Compressibility compensation |
Automatic and continuous |
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Priming |
Wet priming can run at flow rates up to 10 mL/min |
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Pump seal wash |
Standard |
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Primary wetted materials |
316 L stainless-steel, PPS, fluoropolymer, UHMWPE blend, sapphire, ruby, zirconia, DLC, PEEK, and PEEK blend, titanium alloy |
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Injection volume range |
0.1 to 50.0 μL as standard Up to 1000.0 μL with optional extension loops |
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Sample capacity |
768 [2x 384-well plate]; or 96 [2 mL vial holders] |
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Any two of the following: |
48-position, 2.00 mL vial holder (total capacity of 96 vials) 96-well plate 384-well plate 48-position, 0.65 mL micro-centrifuge tube plate 24-position, 1.50 mL micro-centrifuge plate |
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Sample compartment temperature |
4.0–40.0 °C, settable in 0.1 °C increments (optional) |
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Temperature accuracy |
+/- 0.5 °C at the sensor |
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Temperature stability |
+/- 1.0 °C at the sensor |
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Injection needle wash |
Integral, active, and programmable |
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Minimum sample required |
3 μL residual, using total recovery 2 mL vials |
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Accuracy (aspiration) |
+/- 0.2 μL |
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Linearity |
>0.999; 0.2–50.0 μL |
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Precision |
<1.0% RSD from 0.5 to 0.9 μL <0.5% RSD from 1.0 to 4.9 μL <0.25% RSD from 5.0 to 1000.0 μL |
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Sample carryover |
≤0.002% [Caffeine] under UV conditions |
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Advanced capabilities |
Auto-dilution; auto-addition; load ahead |
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Primary wetted materials |
316 L stainless-steel, gold plated stainless-steel, polyimide, PEEK blend, DLC |
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Column capacity |
CM-A: Two columns, as standard (maximum length of 150 mm with filter or guard column) can be supported with optional tubing kit, up to 4.6 mm internal diameter (I.D.) |
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Switching valves |
Two nine-port, eight-position valves; provides programmable, automatic, random-access switching, waste and bypass positions for rapid solvent changeover |
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Column compartment temperature |
4.0 to 90.0 °C, settable in 0.1 °C increments; two independent heat/cool zones per module, up to six zones in stacked configuration |
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Temperature accuracy |
+/- 0.5 °C at the sensor |
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Temperature stability |
+/- 0.3 °C at the sensor |
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Solvent conditioning |
Active pre-heating |
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Column tracking |
eCord Technology tracks column usage and history |
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Column capacity |
Single column, up to 7.8 mm I.D.; up to 300 mm length with filter or guard column |
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Column selection |
Up to three with optional 3-column selection valve (8 port, 9500 psi) |
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Column compartment temperature |
30 cm CH: 20.0 (or 5.0 °C above ambient) to 65.0 °C |
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Temperature accuracy |
+/- 0.5 °C at the sensor |
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Temperature stability |
+/- 0.3 °C at the sensor |
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Solvent conditioning |
Passive pre-heating |
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Informatics compatibility |
Empower Chromatography Data System |
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Communications |
Ethernet |
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Event input/output |
Contact closure and/or TTL input/output |
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Acoustic noise [total system] |
≤65 dBA |
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Operating temperature range |
4.0 to 40.0 °C |
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Operating humidity range |
20% to 80%, non-condensing |
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Power requirements |
100 to 240 VAC |
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Line frequency |
50 to 60 Hz |
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Power consumption |
QSM-R: 200 VAC SM FTN-R: 400 VAC 30 cm CH: 50 W CH30-A: 50 W |
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ACQUITY Arc System: Quaternary Solvent Manager-R, Sample Manager FTN-R, 30-cm Column Heater, and Mounting Bracket |
Width: 57.4 cm (22.6 in.) Height: 57.1 cm (22.5 in.) Depth: 62.8 cm (24.7 in.) Weight: 59.1 kg (130.0 lbs) |
Overview
- Achieve faster set-up with less troubleshooting and more confidence with the ACQUITY Arc MDLC System’s ready-to-use configurations
- Increase resolution by incorporating orthogonal selective columns with heart-cutting MDLC
- Increase sensitivity and selectivity with additional reversed-phase separation
Recommended Use: For heart cutting MDLC where increased resolution is needed.
Features Header
Increase resolution of an elution with heart-cutting functionality
The ACQUITY Arc MDLC System is set up to take a heart cut from the first dimension to another dimension by using a loop trap for peak refocusing, enabling scientists to achieve increased resolution of a specific elution in one dimension and use a different set of conditions on a second separation.
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Purposefully designed for MDLC
The ACQUITY Arc MDLC System is specifically designed for MDLC applications. This system can easily house two columns with different column chemistries (up to 150 mm length) with independent temperature control for each column and operation as well as active solvent pre-heaters, providing versatility for orthogonal separations.
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The ACQUITY Arc MDLC System from Waters is an advanced Multi-Dimensional Liquid Chromatography system that combines exceptional performance, flexibility, and dependability. This state-of-the-art system is designed to tackle the complex demands of modern chromatography, making it a valuable tool for a wide range of applications.
One of the system's most notable features is its ability to separate complex mixtures into their individual components. This multi-dimensional capability is particularly useful for detailed analyses in areas like pharmaceuticals, environmental testing, and food safety. The system ensures high-resolution separations with great reproducibility and sensitivity, which are crucial for obtaining accurate and reliable results.
Flexibility is a key strength of the ACQUITY Arc MDLC System. It can be used with various detectors, columns, and sample types, making it a versatile choice for any lab. The user-friendly interface simplifies operation and method development, making it accessible for both beginners and experienced users, thus boosting lab productivity and efficiency.
Durability and reliability are built into the system, allowing it to perform consistently over time and reducing the need for frequent maintenance. Advanced control and automation features provide precise control over the chromatographic process, enhancing workflow efficiency and minimizing human error. Additionally, the system's advanced detection technologies enable the identification and quantification of a wide range of substances with high sensitivity.
The ACQUITY Arc MDLC System is also scalable, making it suitable for both small-scale research and large-scale industrial applications. It meets regulatory standards and offers comprehensive documentation and data management features, ensuring that analytical results are compliant and easily traceable for audits.
Waters supports the ACQUITY Arc MDLC System with extensive training and technical assistance, helping users to fully utilize its capabilities. This support includes application notes and training programs designed to help users achieve optimal performance.
Ideal for various applications, including pharmaceutical development, environmental analysis, food and beverage testing, and biological and clinical research, the ACQUITY Arc MDLC System offers a blend of superior performance, flexibility, and reliability. It is an excellent choice for any laboratory seeking high-quality analytical results.
FAQs about ACQUITY ARC Multi-Dimensional Liquid Chromatography (MDLC) System
What is the ACQUITY ARC Multi-Dimensional Liquid Chromatography (MDLC) System?
The ACQUITY ARC Multi-Dimensional Liquid Chromatography (MDLC) System is a comprehensive chromatography system that combines high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) with advanced detection capabilities.
What are the benefits of using the ACQUITY ARC MDLC System?
The ACQUITY ARC MDLC System offers a range of benefits, including improved sample throughput, increased sensitivity, and enhanced data quality. It also provides a comprehensive range of detection capabilities, including UV-Vis, fluorescence, and mass spectrometry.
What types of samples can be analyzed with the ACQUITY ARC MDLC System?
The ACQUITY ARC MDLC System can be used to analyze a wide range of samples, including proteins, peptides, nucleic acids, and small molecules.
What type of software is used with the ACQUITY ARC MDLC System?
The ACQUITY ARC MDLC System is compatible with Waters Empower 3 Chromatography Data Software, which provides powerful data analysis and reporting capabilities.
What type of support is available for the ACQUITY ARC MDLC System?
Waters provides comprehensive support for the ACQUITY ARC MDLC System, including installation, training, and technical support.
