ACQUITY Arc Multi-Dimensional Liquid Chromatography System
Multi-dimensional technology for HPLC separations
Waters ACQUITY Arc Multi-Dimensional Liquid Chromatography (MDLC) System enables scientists to achieve significant increase in sensitivity and selectivity, eliminate unwanted interferences, and characterize the most complex samples.
With the system, your lab can perform separations that are normally incompatible with a mass spectrometer by adding a second reversed-phase separation to your experiments. The system is comprised of two (2) Quaternary Solvent Managers (QSM), a Sample Manager with Flow-Through Needle (SM-FTN), and a Column Manager. Additional hardware includes, idiverter valves that are used when choosing the 30 cm Column Heater (CH) product.
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ACQUITY Arc Multi-Dimensional Liquid Chromatography (MDLC) System
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Specifications
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Total system bandspread, 4s |
≤30 μL (default configuration) |
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Dwell volume (total system) |
Path 1≤1450 μL Path 2≤1150 μL |
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Gradient delay volume |
Path 1≤1050 μL Path 2≤750 μL |
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Integrated leak management |
Leak sensors, as standard, and safe leak handling |
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Quantum synchronization |
Injection synchronization between pump and injector enhances retention time reproducibility |
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Operating flow rate range |
0.001 to 5.000 mL/min, in 0.001 mL increments |
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Maximum operating range |
9500 psi up to 5.000 mL/min |
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pH range |
1 to 12.5 |
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Unattended operation |
Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software |
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Cycle time |
≤30 seconds inject-to-inject |
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Solvent capacity |
Blend up to four solvents in any combination (standard); total capacity of nine solvents with integrated solvent select valve (optional) |
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Number of fluidic paths |
Three (Path 1, Path 2, and waste), with Arc Multi-flow path technology (standard) |
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Dwell volume selection |
Automated with Arc Multi-flow path technology |
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Solvent conditioning |
Integrated vacuum degassing, four chambers |
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Gradient formation |
Low-pressure mixing, quaternary gradient |
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Gradient profiles |
11 gradient curves [including linear, step (2), concave (4), and convex (4)] |
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Check valves |
Passive check valves |
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Flow accuracy |
+/- 1.0% at 0.5, 3.0, and 5.0 mL/min |
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Flow precision |
≤0.075% RSD or +/-0.020 min SD, whichever is greater, based on six replicates [60:40 water:methanol pre-mixed; 1.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C18 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm] |
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Composition ripple |
≤0.5 mAU [mobile phase containing 0.1% TFA in water/acetonitrile; 1.5 mL/min; CORTECS C18 2.7 μm, 4.6 x 50 mm; 35 °C; UV @214 nm] |
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Composition accuracy |
+/- 0.5% absolute (full scale) from 5 to 95%; 0.5 to 5.0 mL/min [methanol; methanol with 5.0 mg/mL caffeine step gradient; UV @273 nm] |
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Composition precision |
+/- 0.15% RSD or 0.04 min SD, whichever is greater based on six replicate injections [60:40 water:methanol via Auto•Blend Technology; 0.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C18 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm] |
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Compressibility compensation |
Automatic and continuous |
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Priming |
Wet priming can run at flow rates up to 10 mL/min |
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Pump seal wash |
Standard |
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Primary wetted materials |
316 L stainless-steel, PPS, fluoropolymer, UHMWPE blend, sapphire, ruby, zirconia, DLC, PEEK, and PEEK blend, titanium alloy |
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Injection volume range |
0.1 to 50.0 μL as standard Up to 1000.0 μL with optional extension loops |
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Sample capacity |
768 [2x 384-well plate]; or 96 [2 mL vial holders] |
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Any two of the following: |
48-position, 2.00 mL vial holder (total capacity of 96 vials) 96-well plate 384-well plate 48-position, 0.65 mL micro-centrifuge tube plate 24-position, 1.50 mL micro-centrifuge plate |
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Sample compartment temperature |
4.0–40.0 °C, settable in 0.1 °C increments (optional) |
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Temperature accuracy |
+/- 0.5 °C at the sensor |
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Temperature stability |
+/- 1.0 °C at the sensor |
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Injection needle wash |
Integral, active, and programmable |
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Minimum sample required |
3 μL residual, using total recovery 2 mL vials |
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Accuracy (aspiration) |
+/- 0.2 μL |
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Linearity |
>0.999; 0.2–50.0 μL |
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Precision |
<1.0% RSD from 0.5 to 0.9 μL <0.5% RSD from 1.0 to 4.9 μL <0.25% RSD from 5.0 to 1000.0 μL |
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Sample carryover |
≤0.002% [Caffeine] under UV conditions |
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Advanced capabilities |
Auto-dilution; auto-addition; load ahead |
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Primary wetted materials |
316 L stainless-steel, gold plated stainless-steel, polyimide, PEEK blend, DLC |
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Column capacity |
CM-A: Two columns, as standard (maximum length of 150 mm with filter or guard column) can be supported with optional tubing kit, up to 4.6 mm internal diameter (I.D.) |
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Switching valves |
Two nine-port, eight-position valves; provides programmable, automatic, random-access switching, waste and bypass positions for rapid solvent changeover |
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Column compartment temperature |
4.0 to 90.0 °C, settable in 0.1 °C increments; two independent heat/cool zones per module, up to six zones in stacked configuration |
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Temperature accuracy |
+/- 0.5 °C at the sensor |
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Temperature stability |
+/- 0.3 °C at the sensor |
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Solvent conditioning |
Active pre-heating |
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Column tracking |
eCord Technology tracks column usage and history |
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Column capacity |
Single column, up to 7.8 mm I.D.; up to 300 mm length with filter or guard column |
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Column selection |
Up to three with optional 3-column selection valve (8 port, 9500 psi) |
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Column compartment temperature |
30 cm CH: 20.0 (or 5.0 °C above ambient) to 65.0 °C |
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Temperature accuracy |
+/- 0.5 °C at the sensor |
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Temperature stability |
+/- 0.3 °C at the sensor |
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Solvent conditioning |
Passive pre-heating |
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Informatics compatibility |
Empower Chromatography Data System |
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Communications |
Ethernet |
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Event input/output |
Contact closure and/or TTL input/output |
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Acoustic noise [total system] |
≤65 dBA |
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Operating temperature range |
4.0 to 40.0 °C |
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Operating humidity range |
20% to 80%, non-condensing |
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Power requirements |
100 to 240 VAC |
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Line frequency |
50 to 60 Hz |
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Power consumption |
QSM-R: 200 VAC SM FTN-R: 400 VAC 30 cm CH: 50 W CH30-A: 50 W |
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ACQUITY Arc System: Quaternary Solvent Manager-R, Sample Manager FTN-R, 30-cm Column Heater, and Mounting Bracket |
Width: 57.4 cm (22.6 in.) Height: 57.1 cm (22.5 in.) Depth: 62.8 cm (24.7 in.) Weight: 59.1 kg (130.0 lbs) |
Overview
- Achieve faster set-up with less troubleshooting and more confidence with the ACQUITY Arc MDLC System’s ready-to-use configurations
- Increase resolution by incorporating orthogonal selective columns with heart-cutting MDLC
- Increase sensitivity and selectivity with additional reversed-phase separation
Recommended Use: For heart cutting MDLC where increased resolution is needed.
Features Header
Increase resolution of an elution with heart-cutting functionality
The ACQUITY Arc MDLC System is set up to take a heart cut from the first dimension to another dimension by using a loop trap for peak refocusing, enabling scientists to achieve increased resolution of a specific elution in one dimension and use a different set of conditions on a second separation.
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Purposefully designed for MDLC
The ACQUITY Arc MDLC System is specifically designed for MDLC applications. This system can easily house two columns with different column chemistries (up to 150 mm length) with independent temperature control for each column and operation as well as active solvent pre-heaters, providing versatility for orthogonal separations.
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