• Application Note

Analysis of Food Sugars in MILK Using ACQUITY UPLC BEH Amide Columns

Analysis of Food Sugars in MILK Using ACQUITY UPLC BEH Amide Columns

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstates analysis of food sugars in milk.

Introduction

Compounds analysed in this application include:

1. p-Toluamide

2. Fructose

3. Glucose

4. Sucrose

5. Maltose

6. Lactose

Structures

Experimental

Chromatographic Conditions

Column:

ACQUITY UPLC BEH Amide 2.1 x 100 mm, 1.7 μm

Part Number:

186004801

Mobile Phase A:

80/20 MeCN/H2O with 0.2% triethylamine [TEA]

Mobile Phase B:

30/70 MeCN/H2O with 0.2% triethylamine [TEA]

Flow Rate:

0.13 mL/min

Flow Profile:

90% A/10% B (75% MeCN with 0.2% TEA)

Injection Volume:

1.3 μL (PLNO)

Sample Concentration:

Standards at 1 mg/mL each

Sample Diluent:

50/50 MeCN/H2O

Column Temperature:

35°C

Strong Needle Wash:

20/80 MeCN/H2O (800 μL)

Weak Needle Wash:

75/25 MeCN/H2O (500 μL)

Seal Wash:

50/50 MeCN/H2O

Instrument:

Waters ACQUITY UPLC with ELSD

ELSD Conditions

Gain:

200

Pressure:

40 psi

Drift Tube Temperature:

40 °C

Nebulizer:

Cooling

Data Rate:

10 pps

Filter Time Constant:

Normal

Results and Discussion

WA60118, September 2009

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