• Application Note

Analysis of Nucleotide Phosphates using ACQUITY UPLC BEH Amide Columns

Analysis of Nucleotide Phosphates using ACQUITY UPLC BEH Amide Columns

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights analysis of nucleotide phosphates.

Introduction

Compounds analysed in this application brief :

  1. 2’-Deoxyguanosine-5’-Triphosphate Trisodium Salt Dihydrate (dGTP) 100 μg/mL
  2. Thymidine 5’-monophosphate (TMP) 10 μg/mL
  3. 2’-Deoxyadenosine 5’-monophosphate (dAMP) 10 μg/mL
  4. 2’-Deoxycytidine 5’-monophosphate sodium salt (dCMP) 25 μg/mL
  5. 2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate (dGMP) 15 μg/mL
  6. 2’-Deoxyadenosine 5’-diphosphate sodium salt (dADP) 15 μg/mL

Structures

Experimental

Test Conditions

Column:

ACQUITY UPLC BEH Amide, 2.1 x 150 mm, 1.7 μm

Part Number:

186004802

Isocratic Mobile Phase:

80/20 MeCN/H2O with 2 mM KH2PO4

Flow Rate:

0.5 mL/min

Injection Volume:

5.0 μL (PLNO)

Sample Diluent:

80/20 MeCN/H2O with 10 mM CH3COONH4 and 0.02% CH3COOH

Column Temperature:

25 °C

Weak Needle Wash:

95/5 MeCN/H2O

Detection:

UV @ 254 nm

Sampling Rate:

20 points/sec

Filter Time Constant:

0.2

Instrument:

Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector

Results and Discussion

1. 2’-Deoxyguanosine-5’-Triphosphate Trisodium Salt Dihydrate (dGTP) 100 μg/mL, 2. Thymidine 5’-monophosphate (TMP) 10 μg/mL, 3. 2’-Deoxyadenosine 5’-monophosphate (dAMP) 10 μg/mL, 4. 2’-Deoxycytidine 5’-monophosphate sodium salt (dCMP) 25 μg/mL, 5. 2’-Deoxyguanosine 5’-monophosphate sodium salt hydrate (dGMP) 15 μg/mL, 6. 2’-Deoxyadenosine 5’-diphosphate sodium salt (dADP) 15 μg/mL

WA60097, June 2009

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