Lansoprazole: Isocratic Separation and Degradation by 0.4 N HCl

Column:

SunFire C₁₈ 4.6 x 150 mm, 5.0 μm (p/n: 186002559)

Mobile phase A:

20 mM HCOO-NH₄ +, pH 3.0

Mobile phase B:

Acetonitrile

Isocratic:

as indicated

Flow rate:

1.4 mL/min

Injection volume:

2 μL

Sample Diluent:

75:25 H₂O:ACN

Sample concentration:

350 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Sampling rate:

10 pts/sec

Time Constant:

0.1

Instrument:

Waters Alliance HT 2795, with 2996

Column:

SunFire C₁₈ 4.6 x 150 mm, 5.0 μm (p/n: 186002559)

SunFire C₈ 4.6 x 150 mm, 5.0 μm (p/n: 186002737)

Mobile phase A:

20 mM HCOO-NH₄ +, pH 3.0

Mobile phase B:

Acetonitrile

Isocratic:

as indicated

Flow rate:

1.4 mL/min

Injection volume:

5 μL

Sample Diluent:

50:50 H₂O:ACN

Sample concentration:

2.63 mg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Sampling rate:

5 pts/sec

Time Constant:

1

Instrument:

Waters Alliance HT 2795, with 2996