This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis salicylic acid and related compounds in serum.
The compounds used in this study are –
            
        | 
                     Column:  | 
                     Symmetry Shield RP8, 3.0 x 150 mm, 5 μm  | 
            
| 
                     Guard column:  | 
                     Sentry Guard Column, 3.9 x 20 mm, 5 μm  | 
            
| 
                     Part numbers:  | 
                     Column - WAT094243, Guard - WAT200675  | 
            
| 
                     Mobile phase:  | 
                     1.2% glacial acetic acid/acetonitrile 75:25  | 
            
| 
                     Flow rate:  | 
                     0.6 mL/min  | 
            
| 
                     Injection volume:  | 
                     10 μL of reconstituted porcine serum extract  | 
            
| 
                     Temperature:  | 
                     40 °C  | 
            
| 
                     Detection:  | 
                     UV @ 236 nm  | 
            
            
        
            
        
            
        WA31763.145, June 2003