ACQUITY UPLC M-Class Trap Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
Trap columns allow the scientist to perform complex 2D separations and to enrich and re-focus the sample before eluting to the nano-separation column for the final separation. The 2D Trap Columns are designed for 2D applications where the first dimension utilizes high pH. The unique combination of fritting technologies allows for the high pH compatibility. This allows the trap columns to withstand pressure changes during injection and thus provide superior longevity. This specific offering contains Waters Symmetry 100 Å pore, C18, 5 µm particles. Symmetry silica particles offer on average the highest retention for peptides and are thus most suitable for trapping applications.
The 2D V/M trap columns connect with a V-detail to the main valve and a smaller M-detail fitting to a T-piece or other suitable connection point in the LC system. Due to their design, 2D trap columns are suitable for bi-directional flow.
