RapiZyme Trypsin, Mass Spectrometry (MS) Grade
Achieve quick, clean, and complete protein digestion
Protein digestions for quantitative bioanalysis, biopharmaceutical peptide mapping or bottom-up proteomics are plagued by long digestion times and complex performance trade-offs. Balancing speed and digestion completeness while maintaining high reproducibility, low artifacts, and complete sequence coverage is also a constant challenge. Moreover, productivity during data analysis is often hampered by messy maps with various non-tryptic peaks (missed cleavages, autolysis peaks, non-specific cleavages, and unmatched peaks). Those numerous small, unexpected peaks require time consuming identification, consequently slowing down your critical work.
RapiZyme Trypsin, Mass Spectrometry (MS) Grade enables uncompromising speed and digestion cleanliness through its inordinately high autolysis resistance, high purity, and improved activity. By virtually eliminating the autolysis that plagues even the most advanced trypsin on the market, RapiZyme Trypsin can be used at comparatively high concentrations to achieve faster digestions without resorting to high temperature or sacrificing digestion completeness. Consequently, improving sample turnaround time, lab operations, and most importantly, making critical decisions confidently and quickly.
RapiZyme Trypsin, Mass Spectrometry (MS) Grade
Specifications
Overview
- Achieve efficient 30-minute digestions without resorting to high temperatures by unlocking use of higher enzyme:protein (E:P) ratios with autolysis resistance
- Carry out more productive data analysis through cleaner baselines
- Achieve high sequence coverage for confident characterization and identification
- Attain reproducibility and predictability as a chemically optimized recombinantly expressed porcine sequence trypsin
- Improve stability, vial recovery and activity upon reconstitution for consistent performance by lyophilizing with an LC-MS compatible excipient
- Robust and flexible for use in a variety of workflow-enhancing protocols
Recommended Use: To achieve improved protein digestions for quantitative bioanalysis, biopharmaceutical peptide mapping or bottom-up proteomics.
How RapiZyme works
RapiZyme Trypsin is a chemically optimized recombinant trypsin. Because it is not animal derived, it is free of contaminating chymotrypsin, does not require treatment with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) inhibitors, and thus is free from non-specific digestion associated challenges.
Rapid Digestions
Extensive and homogenous methylation confers RapiZyme Trypsin with inordinately high autolysis resistance. This enables use of RapiZyme Trypsin at high E:P ratios (e.g., 1:5) to drive complete digestions in just 30-minutes, without resorting to higher temperature. Using Remicade® as a case study, RapiZyme Trypsin delivers <0.1% autolysis peaks and <1% missed cleavage (measured as % of total TIC response). An industry leading competitive trypsin delivers 6–7% autolysis peaks and 4–5% missed cleavage under the same conditions.
Clean baselines
Critical decisions are made by recognizing the smallest changes in a map. Regulatory agencies demand identification of every peak. Unmatched peaks, artifact peaks, autolysis peaks, and missed cleavage peaks are all time-consuming and unnecessary noises. They require extra scientific review and analysis, causing software processing times to shoot through the roof when non-tryptic peaks, or autolysis peaks from an in-silico model of trypsin digesting itself are included.
With RapiZyme Trypsin, you can proceed quickly and confidently with exquisite baselines free from noise and artifact peaks. All with a 30-minute digestion.
Reproducibility and purity – what’s in your trypsin?
RapiZyme Trypsin, homogenously methylated, recombinant (non-animal derived) trypsin is free of contaminating chymotrypsin. Further it is lyophilized with a LC-MS compatible excipient to make reconstitution easy and predictable and improve recovery from the vial.
The result is exceptional reproducibility both batch-to-batch and digestion-to-digestion.
Flexible to use
RapiZyme Trypsin can do everything you expect any trypsin (in solution or immobilized) to do and much more. With RapiZyme Trypsin, you can:
- Eliminate desalt & enhance consistency for MAM, PTM monitoring, bioprocessing and proteomics applications. RapiZyme trypsin maintains high activity with modest amounts of guanidine HCl
- Dilute guanidine HCl to 0.5–0.7M and digest in 2 hours at a 1:5 enzyme:protein ratio (pH 7.5)
- Control artifacts for any application when overnight digests are preferred without compromising digestion efficiency
- Digest overnight with a 1:100 enzyme:protein ratio at room temperature and pH 6.5
- Accelerate traditional 1:20 enzyme:protein ratio digests to 1–3 hours to afford additional flexibility
- Heat and eliminate the need for immobilized enzymes for simpler or easier to digest proteins with thermally stable RapiZyme
- Digest with a 1:10 enzyme:protein ratio for 1 hour at 70 °C
