Amino Acid Analysis Standards & Kits
Accurate amino acid composition analysis for HPLC, UHPLC, and UPLC
Accurately separating, identifying, and quantitating amino acids is challenging. The amino acid sequence is the primary structure of a protein and it is unique to a particular peptide or protein. Amino acids are also key ingredients in the cell culture media and feed used to prepare recombinant proteins. Determining amino acid composition in protein/peptide hydrolysates, foods, feeds, and pharmaceutical preparations is important, as regularly monitoring individual amino acid consumption can help you optimize the conditions in the bioreactor.
You can easily analyze amino acids with the many manual and automated solutions that use pre-column derivatization from Waters. With Waters AccQ•Tag Ultra UPLC/UHPLC reagent kit and AccQ•Fluor HPLC reagent kit, you’ll have what your lab needs for accurate amino acid composition analysis.
AccQ•Tag Ultra and AccQ•Fluor derivatization solutions utilize a single reagent to tag both primary and secondary amino acids, reducing reagent usage by 50% compared to competitive kits that require two separate derivatization compounds. This streamlined approach simplifies the gradient and eliminates the need to switch detection wavelengths mid-method.
Amino Acid Analysis Standards & Kits
Specifications
Amino Acid Standards
Amino Acid Kits
Overview
- Achieve stable derivatives with simple, fast, sensitive pre-column derivation using UPLC, UHPLC, or HPLC instrumentation
- Derivatized amino acids resolved and quantified with UV and Fluorescence
- Complete workflow solution including derivatization reagents, columns, eluents, standards, and methods
- Automation enabled on multiple platforms with verified scripts, including Andrew+ Robot and OneLab protocols
- Application-specific amino acid standard kits for identification and quantification
View useful sample preparation workflow guidelines for the hydrolysis and analysis of amino acids from purified peptides/proteins, foods, and feeds in the Comprehensive Guide to Hydrolysis and Analysis of Amino Acids
Recommended Use: For accurately separating, identifying, and quantitating amino acids.
Amino Acid Analysis Manual Kits
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Pre-column derivatization reagents -
Dedicated reversed-phase columns quality control (QC) tested for amino acid analysis
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Eluents and standards
Enable your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, feeds, foods, pharmaceutical preparations, and many other sample matrices. With the ability to run on HPLC, UHPLC, or UPLC, you can have a system that is customized to separate amino acids from protein hydrolysates, cell culture media, and food/feed samples.
Amino Acid Analysis Automation Kits
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High-throughput sample prep (up to 96 samples at a time)
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Minimal user intervention
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Larger reagent volume to accommodate automation needs
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Starter kits include reagents, column, eluents, application standards, and labware
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Scripts ready for multiple automated platforms: Andrew+ Robot, Tecan, and Hamilton
Simplified tools to enhance high throughput amino acid automation via an easy-to-use, automation friendly format that maximizes workflow and accommodates residual volume. Compatible with Andrew+ Robot, Tecan, and Hamilton systems, the AccQ•Tag Ultra Derivatization Automation Kit provides fast, accurate, and precise amino acid analyses, translating into cost and time savings for your lab.
Amino Acid Analysis Reference Standards
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Cell Culture Standard Kit
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Food & Feed Standard Kit
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Amino Acid Hydrolysates Standard
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Norvaline (Internal Standard)
Waters offers reference standards for Amino Acid Analysis (AAA) required in many applications in pharmaceutical and food and feed industries. A variety of standards containing free amino acids are offered for qualitative and quantitative determination of amino acids, method development, and troubleshooting of the AccQ•Tag Ultra and AccQ•Tag methods.
Fast, accurate, quantitative amino acid analysis for protein characterization and cell culture monitoring
Whether you need to determine amino acid composition of a product and compare it to the gene sequence or natural counterpart, or if you are downstream and monitoring cell culture media for critical nutrients to optimize expression efficiencies and make timely product harvesting decisions, AccQ•Tag Ultra amino acid analysis solutions provide rugged and reliable performance to help make accurate decisions about the product throughout an organization.
Robust solution for amino acid analysis in food and feeds
Animal feeds pose several significant challenges for accurate amino acid analysis. Quantitation of amino acids released by hydrolysis is one important measure of nutritional value. A free amino acid profile can identify the origin of a food product, and in that way, detect adulteration. Free amino acids are also metabolic indicators that can be used to monitor and optimize processes such as fermentation. With Waters amino acid analysis solution, you can have confidence that products meet the manufacturing specifications and avoid costly reformulations.
Let Andrew+ Robot pipette for you
One of the largest pain points in sample preparation is repetitive pipetting. Minimizing sources of contamination is also critical. The Andrew+ robotic pipetting platform helps alleviate those pain points and provides confidence and reliability when doing derivatization. With Andrew+ you get flexibility in the number of samples run using AccQ•Tag Ultra Automation Sample Preparation Kit (32, 64, 96 sample), verified protocols available in OneLab, compatibility with additional connected devices, self-guided installation, online troubleshooting, and help.
List of Amino Acids in Each Amino Acid Standard
Amino acid analysis is required in many applications in pharmaceutical and food and feed industries. A variety of standards containing free amino acids are offered for qualitative and quantitative determination of amino acids, method development and troubleshooting of the AccQ•Tag Ultra method.
