AccQ•Tag Columns for Amino Acid Analysis
Accurate amino acid composition analysis using Waters column solutions starts here
Obtaining accurate amino acid analysis (AAA) composition from cell culture media, protein/peptide hydrolysates, dairy and adult nutritional products, feeds and foods, and many other samples require a rigorous and specific approach. For these challenging workflows, Waters offers a comprehensive pre-column derivatization AAA solution, including columns, mobile-phase eluents, sample preparation kits, and amino acid application standards for use with UPLC, UHPLC, and HPLC instrumentation. Using Waters UPLC AAA solutions provides 2x better resolution in ⅓ of the time compared to traditional high-speed post-column derivatization methods for amino acid analysis, allowing you to achieve better results in less time.
The AccQ•Tag Ultra Columns are designed for reversed-phase separation of amino acids derivatized with AccQ•Tag Ultra reagent. Each column is QC tested by UV detection using Waters Amino Acid standard to ensure repeatable performance for your most demanding amino acids. The 1.7 µm, AccQ•Tag Ultra columns are designed for superior speed and resolution on UPLC systems. Waters provides other AccQ•Tag column solutions for both UHPLC and HPLC platforms to ensure broad compatibility.
AccQ•Tag Columns for Amino Acid Analysis
Specifications
Overview
- AccQ•Tag Ultra Columns provide best-in-class column efficiency for superior separation speed, sensitivity, and resolution for amino acid analysis
- These columns are designed for reversed-phase separation of amino acid derivatives produced by the AccQ•Tag Ultra Chemistry Derivatization Kit (Learn more)
- Each AccQ•Tag column is quality control (QC) tested using Waters amino acid standard to ensure repeatable performance for your most demanding amino acids
- View useful workflow guidelines for the hydrolysis and analysis of amino acids from purified peptides/proteins, foods, and feeds in the Comprehensive Guide to Hydrolysis and Analysis of Amino Acids
- Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using ACQUITY stationary phases
Recommended Use: For accurate amino acid composition analysis.
Features Header
AccQ•Tag Ultra Column (1.7 µm) for Waters UPLC Instruments
The AccQ•Tag Ultra Column provides the fastest runs and best resolution for Amino Acid Analysis (AAA). Optimized for Waters H-Class UPLC systems, this solution integrates instrumentation, derivatization chemistry, reversed-phase columns, separation column, eluents, and methods into a seamless, synergistic workflow.
AccQ•Tag Ultra C18 Column (2.5 µm) for UHPLC*
Achieve optimal resolution and shorter run times with AccQ•Tag Ultra 2.5 µm Columns. These columns are certified for use with the AccQ•Tag Ultra Chemistry Kit and AccQ•Tag Ultra Eluents to ensure reliable amino acid analysis.
*Recommended for UHPLC systems running 9,000 psi to 12,000 psi.
AccQ•Tag Column (4 µm) for HPLC
The AccQ•Tag Amino Acid Column is designed and certified for use with the AccQ•Tag method. These reversed-phase HPLC columns separate amino acid derivatives produced by the AccQ•Fluor Reagent Kit with proven performance and consistency.
Legacy Pico•Tag Column (4 µm) for HPLC
The Pico•Tag Column for amino acid analysis is certified for use with the Pico•Tag method and reagent kit. This HPLC column provides trusted separations of amino acid derivatives produced by phenylisothiocyanate (PITC) derivatization.
AccQ•Tag Ultra and AccQ•Fluor Derivatization
The AccQ•Tag Ultra and AccQ•Fluor derivatization methods use pre-column reagents (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) to label amino acids in a simple, single-step reaction, producing highly stable, detectable adducts.
- The AccQ•Tag Ultra Derivatization Kit is optimized for UPLC and UHPLC systems and used with 1.7 µm and 2.5 µm AccQ•Tag Ultra Columns. This kit is QC tested with UV detection.
- The AccQ•Fluor Reagent Kit is designed for HPLC systems and used with the 4 µm AccQ•Tag Amino Acid Column. This kit is QC tested with fluorescence detection.
These kits ensure consistent labeling, streamlined workflows, and reliable results.
Let Andrew+ pipette for you
Improve accuracy, boost reproducibility, minimize errors, and reduce sample prep time with the Andrew+ Pipetting Robot. Save samples and reagents with Andrew+ through flexible sample runs using AccQ•Tag Ultra Automation Sample Preparation Kit (32, 64, 96 sample sets). Benefit from verified OneLab protocols, compatibility with additional connected devices, self-guided installation, and easy access to online troubleshooting and support.
