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Faster Sample Analysis of Fat Soluble Vitamins using CORTECS C8 Columns

Faster Sample Analysis of Fat Soluble Vitamins using CORTECS C8 Columns
  • Kenneth D. Berthelette
  • Thomas Swann
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This technology brief demonstrates how to achieve faster analysis of extremely hydrophobic compounds.

CORTECS UPLC C8 Columns contain solid-core particles with a trifunctionally bonded C8 ligand attached. Similar to columns with a C18 ligand, the CORTECS UPLC Columns offer approximately the same selectivity; however due to the shorter alkyl chain, the CORTECS UPLC C8 Column is less hydrophobic. The decreased hydrophobicity of the column leads to decreased retention times of many compounds, which can advantageous when analyzing extremely hydrophobic analytes such as fat soluble vitamins.

Benefits

  • CORTECS C8 Column chemistry makes analyzing fat soluble vitamins faster and less expensive than the more commonly used C18 Column chemistries.
  • Faster analysis of extremely hydrophobic compounds.

Introduction

Fat soluble vitamins (FSVs), such as vitamin E, are very hydrophobic compounds. Using C18, the most common reverse-phase liquid chromatography column chemistry, to analyze such compounds can lead to lengthy analysis times and higher costs  per run.  

Results and Discussion

CORTECS UPLC C8 Columns can help reduce the analysis time of highly hydrophobic compounds. As an example, Figure 1 shows the analysis of FSVs on a CORTECS UPLC C8 Column vs. a CORTECS UPLC C18 Column. An ACQUITY UPLC H-Class System was used with a mobile phase composition of 90:10 methanol:water at a flow rate of 1.0 mL/min and UV detection at 285 nm. As the figure shows, a CORTECS UPLC C8 Column 1.6 µm, 2.1 x 50 mm (p/n: 186008399) was able to separate the four FSVs approximately four times faster than a CORTECS UPLC C18 Column, 1.6 µm, 2.1 x 50 mm (p/n: 186007093). The decreased hydrophobicity of the CORTECS C8 Column reduces the analysis time and solvent cost for these particular compounds. The difference in hydrophobicity between CORTECS UPLC C8 and CORTECS UPLC C18 Columns can lead to very different chromatography under the same separation conditions.

Figure 1. Analysis of four fat soluble vitamins using a CORTECS UPLC C8 Column, 1.6 µm, 2.1 x 50 mm (p/n 186008399) and a CORTECS UPLC C18 Column, 1.6 µm, 2.1 x 50 mm (p/n 186007093).

Conclusion

CORTECS UPLC C8 Columns contain solid-core particles with a trifunctionally bonded C8 ligand attached. Similar to columns with a C18 ligand, the CORTECS UPLC  Columns offer approximately the same selectivity; however due to the shorter alkyl chain, the CORTECS UPLC C8 Column is less hydrophobic. The decreased hydrophobicity of the column leads to decreased retention times of many compounds, which can advantageous when analyzing extremely hydrophobic analytes such as fat soluble vitamins.

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