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LC-MS Gradient Separation of Buprenorphine and Buprenorphine Glucuronide on XBridge HILIC

LC-MS Gradient Separation of Buprenorphine and Buprenorphine Glucuronide on XBridge HILIC
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the LC-MS gradient separation of buprenorphine and buprenorphine glucuronide on XBridge HILIC Columns.

Introduction

The compounds used in this study are:

  1. Buprenorphine
  2. Buprenorphine glucuronide

Experimental

Method Conditions

Column:

XBridge HILIC, 3.5 μm, 2.1 x 100 mm

Part number:

186004433

Mobile phase A:

10 mM NH4COOH in H2O, 0.125% HCOOH in 50:50 ACN:H2O

Mobile phase B:

10 mM NH4COOH in H2O, 0.125% HCOOH in 90:10 ACN:H2O

Flow rate:

0.3 mL/min

Injection volume:

5 μL

Sample concentration:

50 ng/mL each

Sample diluent:

75:25 ACN:MeOH with 0.2% HCOOH

Column temperature:

30 °C

Instrument:

Waters ACQUITY UPLC with TQD

MRM:

Buprenorphine 468.3 > 54.7 Buprenorphine glucuronide 644.3 > 468.3

Dwell time:

75 ms

ISD:

10 ms

ICD:

10 ms

Gradient:

Time(min)

Profile

%A

%B

0.00

0.1

99.9

1.05

0.1

99.9

4.35

99.9

0.1

5.00

99.9

0.1

5.01

0.1

99.9

6.00

0.1

99.9

Results and Discussion

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WA64077, August 2009

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