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Oxytocin and Angiotensins in Serum

Oxytocin and Angiotensins in Serum
  • Waters Corporation
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Plasma preparation for centrifuge

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of oxytocin and angiotensins in serum using SymmetryShield RP8 and Sentry guard column.

Introduction

Compouns used in this application brief includes:

1. Oxytocin

2. Angiotensin II

3. Angiotensin I (I.S.)

Experimental

HPLC Method

Column:

SymmetryShield RP8, 4.6 x 100 mm, 3.5 μm (p/n: WAT094226)

Guard Column:

Sentry guard column 3.9 x 20 mm, 3.5 μm (p/n: 186000704)

Mobile phase A:

0.1% TFA in water

Mobile phase B:

Acetonitrile/water 90:10 with 0.1% TFA

Flow rate:

1.4 mL/min

Injection volume:

30 μL of reconstituted porcine extract

Temperature:

30° C

Detection:

UV @ 227 nm

Gradient

Time (min)

%A

%B

0

85

15

22

40

60

Results and Discussion

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WA31763.115, June 2003

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