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Acetaminophen and Codeine

Acetaminophen and Codeine
  • Waters Corporation
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Closeup of woman pouring a bunch of pills from a bottle into her hand

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of acetaminophen and codeine using Symmetry C8 columns.

Introduction

Compounds analyzed in this study are:

1. Acetaminophen

2. Codeine

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 μm (p/n: WAT046970)

Mobile phase:

10 mM docusate sodium in methanol/water/THF/phosphoric acid 60:36:4:1

Flow rate:

0.75 mL/min

Injection volume:

15 μL of 55 μg/mL acetaminophen and 35 μg of codeine sample

Detection:

UV @ 254 nm

USP Tailing Factors

1. 1.09
2. 1.04 

Results and Discussion

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WA31763.11, June 2003

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