• Application Note

Insulin- Isophase Suspension

Insulin- Isophase Suspension
  • Waters Corporation
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Young man holding insulin and syringe

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of insulin- isophase suspension.

Introduction

The compounds used in this study are –

Compounds

USP Tailing Factors

Unknown

1.1

Insulin A and B chains with 51 residues, 5733 daltons

1.2

Unknown

1.2

Experimental

HPLC Method

Column:

Symmetry C18, 4.6 x 150 mm, 5 μm

Part number:

WAT045905

Mobile phase A :

0.1% TFA in Water

Mobile phase B :

Acetonitrile with 0.1% TFA

Flow rate:

1.0 mL/min

Injection volume:

5 μL of sample with 150 μL of insulin suspension and 300 μL of 0.01N HCL

Suspension contains zinc-insulin crystals and protamine sulfate in buffered waters

Detection:

UV @ 280 nm

Gradient

Gradient.

Results and Discussion

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WA31763.93, June 2003

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