• Application Note

Tricyclic Antidepressants - Isocratic, pH 9.0, XTerra Phenyl

Tricyclic Antidepressants - Isocratic, pH 9.0, XTerra Phenyl
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of tricyclic antidepressants using XTerra Phenyl Columns. 

Introduction

The compounds analyzed in this study are: 

  1. Nordoxepin 
  2. Nortriptyline 
  3. Doxepin 
  4. Imipramine 
  5. Amitriptyline 
  6. Trimipramine 
Compounds

Experimental

Conditions

Column:

XTerra Phenyl, 4.6 x 100 mm, 3.5 µm

Part number:

186001139

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

100 mM NH4HCO3 in H2O, pH 9.0

Flow rate:

1.4 mL/min

Isocratic mobile phase composition:

25% A; 65% B; 10% C

Injection volume:

20 µL (66 µg/mL)

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Results and Discussion

Results

Featured Products

WA20738.109, June 2009

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