• Application Note

25mer Oligonucleotide Purification (0.5 μmole scale)

25mer Oligonucleotide Purification (0.5 μmole scale)
  • Waters Corporation
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Oligonucleotide illustration

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the oligonucleotide purification using XTerra C18 columns.

Introduction

25mer oligonucleotide has been analyzed in this application brief.

Experimental

HPLC Method

Column:

XTerra MS C18 4.6 x 50 mm, 2.5 μm (p/n: 186000602)

Mobile phase A:

5% ACN, 95% 100 mM Triethylammonium acetate, pH 7.0

Mobile phase B:

30% ACN, 70% 100 mM Triethylammonium acetate, pH 7.0

Flow rate:

1.0 mL/min

Injection volume:

80 μL

Temperature:

60°C

Detection:

UV @ 260 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient

Time (min)

%A

%B

0

80

20

15

42.5

57.5

Results and Discussion

Featured Products

WA20738.002, June 2002

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