• Application Note

Microcystins in Natural Waters

Microcystins in Natural Waters
  • Waters Corporation
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Green algae on the surface of water

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

Microcystin-LR is a potent mammalian toxin which is known to have been responsible for the deaths of domesticated animals, livestock loss, and the potential presence in potable water supplies.

Introduction

Microcystin-LR is a potent mammalian toxin which is known to have been responsible for the deaths of domesticated animals, livestock loss, and the potential presence in potable water supplies.

Experimental

Pretreatment

1. Filter water sample through 0.45 μm membrane filter.

2. Add 100 μL of enkephalin (concentration 10 μg/L) to 10 mL filtered water sample and mix thoroughly.

SPE Procedure

LC Conditions

System:

Alliance HPLC 2695

Column:

Symmetry300 C18, 3.5 μm, 4.6 x 75 mm

Flow rate:

0.2 mL/min

Mobile phase A:

0.2% formic acid in water

Mobile phase B:

0.2% formic acid in methanol

Injection volume:

10 μL

Column temp.:

30 °C

Gradient

MS Conditions

MS System:

Waters Quattro Ultima Pt

Ionization mode:

Positive electrospray (ESI+)

Multiple reaction monitoring

Results and Discussion

Recovery data
Recovery data for spiked samples at various concentrations. 

References

  1. Determination of Microcystins in Natural Water by Liquid Chromatography Tandem Mass Spectrometry, Chen Qi, Huang Baifen, Zhang Jing, Ren Yiping; Zhejiang Provincial Center for Disease Prevention and Control.

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720002595, April 2008

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