• Application Note

Antidepressants in Plasma

Antidepressants in Plasma
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of antidepressants in plasma using Symmetry Columns. 

Introduction

 The compounds analyzed in this study are:

  1. Nordoxepin 
  2. Nortriptyline 
  3. Norverapamil 
  4. Doxepin
  5. Amitriptyline 
  6. Verapamil 
  7. Methoxyverapamil (I.S.) 

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.9 x 150 mm, 5 µm

Part number:

WAT200655

Mobile phase:

100 mM phosphate pH 7.0/acetonitrile/methanol 55:35:10

Flow rate:

1.0 mL/min

Injection volume:

100 µL acidified plasma sample solution

Temperature:

30 °C

Detection:

UV @ 230 nm

Results and Discussion

Chromatogram of Plasma Extracts: A) Blank B) Spiked Sample
Chromatogram of Plasma Extracts: A) Blank B) Spiked Sample.

Featured Products

WA31763.29, June 2003

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