• Application Note

Analysis of Aromatic Amino Acids Using Atlantis T3

Analysis of Aromatic Amino Acids Using Atlantis T3
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of aromatic amino acids.

Introduction

Compounds analysed in this application brief :

  1. Tyrosine
  2. Phenylalanine
  3. Tryptophan

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 50 mm, 3 μm

Part Number:

186003726

Mobile Phase A:

H2O

Mobile Phase B:

MeOH

Mobile Phase C:

100 mM HCOONH4, pH 3.0

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Sample:

Tyrosine (150 μg/ mL), Phenylalanine (200 μg/mL), Trytophan (30 μg/mL) in H2O

Column Temperature:

30 ˚C

Sample Temperature:

20 ˚C

Detection:

UV @ 254 nm

Sampling Rate:

5 point/sec

Filter Response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Gradient

Time (min)

Profile

%A

%B

%C

0.00

90

0

10

10.00

10

80

10

11.00

90

0

10

15.00

90

0

10

Results and Discussion

1. Tyrosine, 2. Phenylalanine, 3. Tryptophan

Featured Products

WA60221, January 2009

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