• Application Note

ACQUITY UPLC Analysis of Acrylamide, Methacrylic Acid, and Methacrylamide

ACQUITY UPLC Analysis of Acrylamide, Methacrylic Acid, and Methacrylamide
  • Waters Corporation
Download PDF Download PDF

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of acrylamide, methacrylic acid and methacrylamide on ACQUITY UPLC BEH Amide Columns.

Introduction

Structures

Structures

Experimental

Test Conditions

Columns:

ACQUITY UPLC BEH Amide, 2.1 x 150 mm, 1.7 μm

Part Number:

186004802

Isocratic Mobile Phase:

95/2.5/2.5 MeCN/IPA/H2O with 5 mM CH3COONH4 and 0.02% NH4OH, pH 9.0

Flow Rate:

0.5 mL/min

Injection Volume:

5.0 μL (PLNO)

Sample Concentration:

30 μg/mL each

Sample Diluent:

75/25 MeCN/MeOH with 0.2% HCOOH

Column Temperature:

25 °C

Weak Needle Wash:

95/5 MeCN/H2O

Detection:

UV @ 210 nm

Sampling Rate:

20 points/sec

Filter Time Constant:

0.2

Instrument:

Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector

Results and Discussion

The compounds analysed in this study are:

  1. Methacrylamide
  2. Acrylamide
  3. Methacrylic acid
Results

Featured Products

WA60108, June 2009

Back To Top Back To Top