• Application Note

Lifetime of Symmetry 3.5 µm Column: 10,000 Injections of Sulfa Drugs

Lifetime of Symmetry 3.5 µm Column: 10,000 Injections of Sulfa Drugs
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the analysis of sulfa drugs.

Introduction

The compounds analyzed in this study are:

  1. Sulfanilamide 
  2. Sulfadiazine 
  3. Sulfathiazole 
  4. Sulfamerazine 
  5. Sulfamethazine 
  6. Succinylsulfathiazole 
Compounds

Experimental

HPLC Method

Column:

Symmetry 3.5µm C18, 4.6 x 100 mm

Guard Column:

Symmetry Guard column, 3.9 x 20 mm, 5 µm

Part numbers:

Column - WAT066220, Guard - WAT054225

Mobile phase:

Water/methanol/glacial acetic acid 79:20:1

Flow rate:

1.5 mL/min

Injection volume:

10 µL of mixture of six sulfa drugs at 10 to 39 µg/mL

Detection:

UV @ 254 nm

Results and Discussion

Featured Products

WA31763.96, June 2003

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