• Application Note

Insulin- Impurity Isolation, Transfer from Analytical to Prep

Insulin- Impurity Isolation, Transfer from Analytical to Prep
  • Waters Corporation
Download PDF Download PDF
Young man holding insulin and syringe

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of insulin- impurity isolation, transfer from analytical to prep.

Introduction

The compounds used in this study are –

  1. Insulin A and B chains with 51 residues, 5733 daltons
  2. Impurity

Experimental

HPLC Method

Columns:

A. Symmetry C18, 4.6 x 150 mm, 5 μm

B. Symmetry Prep C18, 7.8 x 300 mm, 7 μm

C. Symmetry Prep C18, 19 x 150 mm, 7 μm

Part numbers:

A. WAT045905

B. WAT066235

C. WAT066240

Mobile phase:

A. 0.1% TFA in Water

B. 0.1% TFA/acetonitrile 26% B to 33% B in 14 minutes adjusted for gradient delay on prep system

Flow rates:

A. 1.0 mL/min b. 5.76 mL/min c. 17mL/min

Detection:

UV @ 280 nm

Sample load:

Bovine pancreas insulin, 10 mg/mL

A. 0.07 mg B. 3 mg C. 8.5mg

HPLC systems:

A. analytical system B. and C. prep system with 0.04 in. I.D. tubing

Results and Discussion

Featured Products

WA31763.92, June 2003

Back To Top Back To Top