• Application Note

Multi-Residue Analysis of Pesticides in Oranges Using AOAC QuEChERS Method by UPLC-MS/MS

Multi-Residue Analysis of Pesticides in Oranges Using AOAC QuEChERS Method by UPLC-MS/MS

  • Waters Corporation
Orange juice pouring into glass

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the multi-residue analysis of pesticides in oranges using AOAC QuEChERS method by UPLC-MS/MS

Experimental

Extraction Procedure

  1. Add 15 mL 1% acetic acid in acetonitrile into the 50 mL DisQuE extraction tube 1.
  2. Add 15 g of homogenized orange with skin into the 50 mL tube.
  3. Add any internal standards and standard mixture.
  4. Shake vigorously for 1 minute and centrifuge > 1500 rcf for 5 minute.
  5. Transfer 1 mL of the acetonitrile extract into the 2 mL clean-up tube containing 50 mg PSA, 150 mg MgSO4, and 50 mg C18.
  6. Shake for 30 seconds and centrifuge >1500 rcf for 1 minute.
  7. Transfer 100 μL of final extract into a 1.5 mL centrifuge tube.
  8. Add any post-extraction internal standards.
  9. Dilute as needed with an appropriate buffer or solvent.
  10. Centrifuge > 16000 rcf for 5 minutes.
  11. Transfer to autosampler vial.

LC Conditions

LC System:

Waters ACQUITY UPLC System

Column:

ACQUITY UPLC BEH C18, 2.1 x 100 mm, 1.7 μm

Column Temp:

40 °C

Sample Temp:

4 °C

Flow Rate:

0.3 mL/min.

Mobile Phase A:

Water + 0.1% formic acid

Mobile Phase B:

Methanol + 0.1% formic acid

Injection Volume:

15 μL, Partial loop injection

Gradient:

Time

Flow Rate

A%

B%

0.00

0.3

75

25

0.25

0.3

75

25

7.75

0.3

5

100

8.50

0.3

0

100

8.51

0.5

75

25

10.50

0.5

75

25

11.00

0.3

75

25

MS Conditions

Instrument:

Waters ACQUITY TQ Detector

Ionization:

Positive electrospray (ESI+)

Acquisition:

Multiple reaction monitoring (MRM)

Results and Discussion

Pesticides in Oranges by UPLC-MS/MS Pesticides in Oranges by UPLC-MS/MS

720003366, March 2010

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