• Application Note

Multi-Residue Analysis of Pesticides in Grapes Using AOAC QuEChERS Method by GC-MS

Multi-Residue Analysis of Pesticides in Grapes Using AOAC QuEChERS Method by GC-MS

  • Waters Corporation
Grapes hanging on vine

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates multi-residue analysis of pesticides in grapes using AOAC QuEChERS method by GC-MS.

Experimental

Extraction Procedure

  1. Add 15 mL 1% acetic acid in acetonitrile into the 50 mL DisQuE extraction tube 1.
  2. Add 15 g of homogenized sample into the 50 mL tube.
  3. Add any internal standards and standard mixture.
  4. Shake vigorously for 1 minute and centrifuge > 1500 rcf for 5 minute.
  5. Transfer 1 mL of the acetonitrile extract into the clean-up tube 2.
  6. Shake for 30 seconds and centrifuge >1500 rcf for 1 minute.
  7. Transfer 0.5 mL extract into a tube.
  8. Add any post-extraction internal standards.
  9. Add 0.25 mL toluene.
  10. Evaporate at 50 °C with N2 to < 0.1 mL.
  11. Bring volume up to 0.2 mL with toluene.
  12. Transfer to vial with insert for analysis.

Test Conditions

GC Conditions

Instrument:

Agilent 6890N GC

Column:

RTX-5MS, 30 x 0.25 mm, (0.25 μm film)

Carrier Gas:

Helium

Flow Rate:

1.0 mL/min

Temp. Program:

Initial 100 °C, hold 1 min, then 10 °C/min to 320 °C, hold for 7 minute

Injection Volume:

2 μL splitless

MS Conditions

Instrument:

Waters Quattro micro GC-MS

Ionization:

Electron Impact (70 eV)

Acquisition:

Single Ion Recording (SIR) Mode

Results and Discussion

Pesticide Recovery in Grapes by GC-MS

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720003370, March 2010

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