• Application Note

Analysis of Anesthetics Using Atlantis T3 Columns

Analysis of Anesthetics Using Atlantis T3 Columns
  • Waters Corporation
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Abstract

This application brief demonstrates analysis of anesthetics using Atlantis T3 Columns.

Introduction

The compunds used in this study are -

  1. Procaine
  2. Lidocaine
  3. Butacaine
  4. Benzocaine
  5. Tetracaine

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 150 mm, 5 μm

Part number:

186003747

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

100 mM HCOONH4, pH 3.75

Flow rate:

1.4 mL/min

Injection volume:

5 μL

Sample:

Procaine (30 μg/mL),

Lidocaine (100 μg/mL),

Butacine (60 μg/mL)

Benzocaine(30 μg/mL),

Tetracaine (60 μg/mL) in H2O

Column temperature:

30 ˚C

Sample temperature:

15 ˚C

Detection:

UV @ 254 nm

Sampling rate:

5 point/sec

Filter response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Gradient

Time(min)

Profile

%A

%B

%C

0.00

65

25

10

5.00

40

50

10

10.00

40

50

10

11.00

65

25

10

15.00

65

25

10

Results and Discussion

Featured Products

WA60218, January 2009

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